摘要
目的:分析耐碳青霉烯类肠杆菌科细菌(CRE)的耐药情况及其耐药基因,为临床防治CRE感染提供依据。方法:收集肠杆菌科细菌6 321株,采用PCR检测技术检测KPC、IMP、VIM,并测序分析基因型别。结果:经检测,6 321株肠杆菌科细菌中含有耐碳青霉烯类肠杆菌科细菌33株,检出率0.52%,在尿液、血液、痰液、胆汁、脓液中均有分布,其中痰液分布最多,为12株。耐碳青霉烯类肠杆菌科细菌是多药耐药菌,对不同药物具有不同程度耐药率,其对氨苄西林、头孢唑啉、舒巴坦耐药率较高,分别为100.00%、90.91%、78.79%。结论:KPC和IMP为产碳青霉烯酶主要基因型,临床加强对耐碳青霉烯类肠杆菌科细菌产碳青霉烯酶的监测具有重要意义。
Objective:To analyze the drug resistance of carbapenem-resistant enterobacteriaceae(CRE)and its drug resistance gene,to provide basis for clinical prevention and treatment of CRE infection..Methods:6 321 strains of enterobacteriaceae were collected.PCR detection technology was used for KPC,IMP,VIM,and gene type was sequencing analyzed.Results:Among thedetection of 6 321 strains of enterobacteriaceae bacteria,there were 33 strains of carbapenem-resistant enterobacteriaceae,and the detection rate was 0.52%.They were distributed in urine,blood,sputum, pus,bile,most of them distributed in sputum with 12 strains.Carbapenem-resistant Enterobacteriaceae were multidrug-resistant bacteria;different drugs had varying degrees of resistance;the resistance rates to ampicillin,cefazolin and sulbactam were 100.00% ,90.91% ,78.79% .Conclusion:KPC and IMP were the maingenotypes of carbapenemases,to strength the monitoring of carbapenemases production of carbapenem-resistant enterobacteriaceae in clinical is importment.
作者
张丽娟
Zhang Lijuan(Department of Laboratory,the People's Hospital of Chongzuo City 532200)
出处
《中国社区医师》
2017年第3期5-5,7,共2页
Chinese Community Doctors
