摘要
为研究生活垃圾填埋场对河流抗生素抗性基因的影响,采用高通量荧光定量PCR技术对厦门市某垃圾填埋场附近河流水中的抗生素抗性基因的多样性和丰度进行了分析.结果表明,垃圾填埋场背景点河水检测到了57种抗性基因,下游河流水检测到了158种抗性基因,下游河流150种抗性基因显著富集(P<0.05),其中增加倍数最大的是磺胺类抗生素抗性基因dfrA1,达2299倍,沿河而下,特别是在垃圾填埋场下游河流水中,抗生素抗性基因检出率、富集倍数都显著增加,抗性基因污染加剧;可移动元件与抗性基因显著相关;生活垃圾填埋场可能对河流水抗生素抗性基因的多样性和丰度产生了影响.
Aquatic environment especially the river is an important reservoir of antibiotic resistance genes(ARGs).Forthe further insight into the ARGs in a river in the vicinity of a landfill,high-throughput qPCR technique was used toinvestigate the diversity and abundance of ARGs.The results showed that a total of57ARGs was detected in controlsample,while,158ARGs was detected in the downstream river sample,in which150ARGs in the downstream river weresignificantly enriched(P<0.05).A maximum enrichment of2299-fold was detected in dfrA1,which conferred resistanceto sulfonamide.The detection number of ARGs and the enrichment of ARGs were significantly increased along the river.The abundance of mobile genetic elements was significantly correlated with the abundance of ARGs.These resultsimplied that landfill may lead to the dissemination and enrichment of ARGs in river.
作者
黄福义
安新丽
李力
欧阳纬莹
李虎
苏建强
HUANG Fu-yi;AN Xin-li;LI Li;OUYANG Wei-ying;LI Hu;SU Jian-qiang(Key Laboratory of Urban Environment and Health,Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China;Lijiang Environmental Monitoring Station, Lijiang 674100, China)
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2017年第1期203-209,共7页
China Environmental Science
基金
国家重点研发计划(2016YFD0800205)
国家自然科学基金国际(地区)合作交流项目(21210008)
