摘要
目的 建立 HPL C法测定清肝颗粒中绿原酸含量的方法。方法 采用 Nova-pak C1 8色谱柱 ,流动相 :甲醇 -冰醋酸 -三乙胺 (14∶ 86∶ 1∶ 0 .3 ) ,流速 :1ml· min- 1 ,检测波长 :3 2 7nm,室温下对清肝颗粒中的绿原酸进行含量测定。结果 绿原酸在 0 .2 5 6～ 0 .5 12μg范围内 ,线性关系良好 (r=0 .99981) ;平均加样回收率为 99.5 3 % ,RSD为 1.5 2 % (n=6)。结论 本方法准确 ,简便 ,灵敏度高 ,结论可靠 ,可作为清肝颗粒的质量控制标准。
OBJECTIVE To establish a RP HPLC method for drtermination of the content of chlorogenic acid in Qinggan Keli.METHODS Nova-pakC 18 column was used,the mobile phase was methanol water aceticaci triethylamine(14∶86∶1∶0.3)at a flow rate of 1ml·min -1 ,the detection wacelength was 327nm and column temperature was room temperature.RESULTS The calibration curve was linear(r=0.99981)in the range of 0.0256-0.512mg for chlorogenic average recovery was 99.53% and RSD% was 1.52(n=6).CONCLUSION The method is accurate,sample,sensitive and reliable and it can be used as the qualily control of Qinggan Keli.
出处
《海峡药学》
2002年第5期43-45,共3页
Strait Pharmaceutical Journal
