摘要
目的 探讨己酮可可碱 (pentoxifyline ,PTX)对NOD(non obesediabetic)小鼠 1型糖尿病的影响及其机制。方法 采用动物模型NOD鼠 ,PTX处理后检测血糖、尿糖及糖尿病发病率 ,HE染色观察胰岛炎 ,并用RT PCR法检测胰腺干扰素γ(IFN γ)、肿瘤坏死因子α(TNF α)、白介素 10 (IL 10 )mRNA的表达。结果 PTX组糖尿病发生率 (30 .0 % )低于对照组 (6 7.9% ) ,P <0 .0 1;胰岛炎程度也减轻P <0 .0 0 1;胰腺IFN γ、TNF αmRNA的表达较对照组降低 ,P <0 .0 5 ;3项变化均具显著性。而IL 10mRNA的表达则无显著改变。结论 PTX可预防NOD小鼠发生糖尿病 ,其机制可能与纠正Th1与Th2型细胞因子比例失衡有关。
Objective To study the effect of phosphodiesterase inhibitor pentoxifylline (PTX) on type 1 diabetes in NOD mice and its possible mechanism.Methods Blood glucose, urinary glucose, insulitis and incidence of diabetes were investigated in NOD mice treated with PTX, insulitis was observed by HE staining and the expressions of IFN γ, TNF α, IL 10 in pancreas were measured by RT PCR technique. Results The incidence of diabetes in the PTX group was 30.0% which was significantly lower than 67.9% in the control group (P<0.01), and the degree of insulitis in the PTX group was also less than that in the control group (P<0.001). IFN γ and TNF α mRNA expressions in pancreas of PTX group were significantly downregulated (P<0.05) and IL 10 mRNA expression was unaffected as compared with the control group. Conclusion PTX prevents NOD mice from developing type 1 diabetes, which may be related to regulating Th1/Th2 cytokines imbalance.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2002年第5期388-390,共3页
Chinese Journal of Endocrinology and Metabolism
基金
山西省回国留学人员科研资助项目资助 (99 94)
关键词
1型糖尿病
己酮可可碱
近交NOD
干扰素Ⅱ
肿瘤坏死因子
白细胞介素10
动物模型
Pentoxifylline
Mice, inbred NOD
Diabetes mellitus, insulin dependent
Interferon type Ⅱ (Interferon γ)
Tumor necrosis factors
Interleukin 10
