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系统性红斑狼疮自身抗体的检测 被引量:2

Detection of Autoantibodies in Systemic Lupus Erythematosus
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摘要 目的 对系统性红斑狼疮和正常人进行抗核抗体 (ANA)、抗双链DNA(ds -DNA)和抗ENA抗体的测定。方法 采用间接免疫荧光法检测ANA ;采用胶体金技术检测抗ds -DNA ;采用免疫印迹技术检测抗ENA抗体。结果 ANA阳性率最高 (96 % ) ;抗ds -DNA阳性率较低 (5 4% )。SLE患者血清中存在多种自身抗体。结论 SLE患者自身抗体的测定对自身免疫性疾病的诊断和分型具有重要意义。 Objective To detect ANA,ds-DNA and anti-ENA antibody of SLE patients and control bodies.Methods ANA was detected by immunofluorescence assay,ds-DNA was detected by colloidal gold method,Anti-ENA antibody was detected by immunoblot method.Results The positive rate of ANA was the highest(96%).While the positive rate of ds-DNA was lover(54%).There were more than one autoantibodies in patient's serum with SLE.Conclusion Detection of autoantibodies in SLE was important to diagnosis and typing. 〔
出处 《深圳中西医结合杂志》 2002年第5期278-279,共2页 Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
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  • 1顾元 赵经飙.用对流免疫扩散电泳法检测ENA抗体的初步总结[J].临床皮肤科杂志,1988,(6):292-292.
  • 2刘敏,冯柏庄,俞纯山.系统性红斑狼疮患者自身抗体检测方法的应用与评价[J].陕西医学检验,1997,12(1):3-5. 被引量:49
  • 3Tan EM ,Cohen AS,Fries JF,et al.The 1982 revised criteria for the class ification of systemic lupus erythematosus [J]. Arthritis Rheum, 1982,25:1271.
  • 4王兰兰.临床免疫学及免疫检验.3版.北京:人民卫生出版社,2003:190-199.
  • 5Gonzalez C,Guevara P,Alarcon I,et al.Antinuclear antibodies (ANA) screening by enzyme immunoassay with nuclear HEp-2cell extract and recombinant antigens:analytical and clinical evaluation.Clin Biochem,2002,35:463-469.
  • 6Bernardini S,Infantino M,Bellincampi L,et al.Screening of antinuclear antibodies:comparison between enzyme immunoassay based on nuclear homogenates,purified or recombinant antigens and immunofluorescence assay.Clin Chem Lab Med,2004,42:1155-1160.
  • 7Emlen W,O' Neill L.Clinical significance of antinuclear antibodies:comparison of detection with immunofluorescence and enzyme-linked immunosorbent assays.Arthritis Rheum,1997,40:1612-1618.
  • 8Ulvestad E,Kanestrom A,Madland TM,et al.Evaluation of Diagnostic Tests for Antinuclear Antibodies in Rheumatological Practice.Scand J Immunol,2000,52:309-315.
  • 9Homburger HA,Cahen YD,Griffiths J,et al.Detection of antinuclear antibodies comparative Evaluation of enzyme immunoassay and indirect immunofluorescence methods.Arch Pathol Lab Med,1998,122:993-999.
  • 10Salonen EM,Miettinen A,Walle TK,et al.Anti-telomere antibodies in systemic lupus erythematosus (SLE):a comparison with five antinuclear antibody assays in 430 patients with SLE and other rheumatic diseases.Ann Rheum Dis,2004,63:1250-1254.

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