摘要
目的 研究碱性成纤维细胞生长因子 (bFGF)对人毛囊黑素细胞 (Hairfolloclemelanocyte ,HFM )增殖活化和黑素合成的影响。方法 体外培养正常人HFM ,观察不同浓度bFGF(0 3～ 1 2ng ml)对HFM形态、增殖、酪氨酸酶活性和黑素含量的影响。结果 bFGF处理HFM 7d后 ,HFM树突增多延长 ,酪氨酸酶活性和黑素含量增加显著高于对照组 (P <0 0 1) ,以 0 6ng ml组为著。结论 bFGF能诱导HFM增殖。
Objective To study the effects of basic fibroblast growth factor (bFGF) on the activation, proliferation and melanin synthesis of cultured human hair follicle melanocytes(HFM). Methods Normal human hair follicle melanocytes were isolated and cultured till logarithmic period of second passage, and then different concentrations of bFGF (0.3～1.2 ng/ml) were added to study the effects on cellular morphologic changes, proliferation, tyrosinase activity and melanin contents of cultured HFM. Results In the cells after 7 days' bFGF treatment, the dendrites were elongated and enriched, and tyrosinase activity and melanin contents were increased compared with those of untreated cells ( P <0.01), especially when the concentration is 0.6 ng/ml. Conclusion bFGF can induce HFM proliferation, tyrosinase activity and melanin synthesis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第10期1245-1247,共3页
Journal of Third Military Medical University
