摘要
目的 研究负载胃癌酸洗抗原树突状细胞 (DC)诱导的特异性CTL对胃癌细胞的杀伤效应。方法 酸洗涤法获得SGC790 1细胞膜抗原多肽 ,GM CSF、IL 4和TNF a体外诱导扩增DC并负载酸洗抗原 ,制备胃癌抗原特异性CTL ;用CytoTox 96TM检测其对SGC790 1体外杀伤效应。结果 负载胃癌抗原肽的DC诱导的特异性CTL对SGC790 1的杀伤率达 88 95 % ,显著高于LAK细胞的杀伤率 (P <0 0 5 )。且其对同种不同分化类型的胃癌细胞株MGC80 3、MNK2 8也有较高的杀伤效应 ,而对LOVO及HepG2肿瘤细胞株无显著杀伤作用 (P >0 0 5 )。结论 负载胃癌抗原的DC体外可诱导出高效而特异的抗胃癌效应 ,提示以DC为中心的肿瘤生物治疗可望提高胃癌综合治疗水平。
Objective To explore the cytotoxic effect of tumor specific cytotoxic T lymphocyte (CTL) induced by gastric cancer peptide pulsed DC on gastric cancer cells. Methods Peptide of gastric cancer strain (SGC7901) was obtained by citrate phosphate buffer elution . Peripheral blood mononuclear cell (PBMC) were cultured under the condition of GM CSF(1 000 U/ml)+IL 4(1 000 U/ml) + TNF α(100 ng/ml). The tumor antigen specific CTL was generated from activated autologous T cell by acid eluted peptide pulsed DC. And the cytotoxicity of CTL to SGC7901 was assayed by Cyto Tox96 TM kit in vitro . Different tumor cell lines and LAK cells were used as control in these experiments. Results The killing rate of SGC7901 by the antigen specific CTL reached at 88.95%, nearly twice as high as that of control . The CTL had much higher cytotoxicity to different differentiated type of gastric cancer cells such as MGC803 and MNK28, as compared with LOVO and HepG2 tumor cells( P <0 01) . Conclusion DC might have potential value in clinical biotherapy of gastric cancers.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第10期1236-1238,共3页
Journal of Third Military Medical University
