期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

hERR1在乳腺癌中的表达及其对芳香族化酶表达的调控 被引量：4

Expression and regulation on aromatase gene of hERR1 in breast cancer
暂未订购
导出
摘要 目的　了解hERR1在乳腺癌及癌旁组织中的表达情况 ,以及它对芳香族化酶基因表达的调节作用。方法　收集乳腺癌患者的临床标本 ,应用RT PCR法 ,研究癌组织及相应的癌旁组织中hERR1的表达情况 ;采用瞬时转染和CAT活性分析的方法 ,研究hERR1对芳香族化酶基因表达的调节。结果　hERR1在癌组织中的表达明显高于相应的癌旁组织 (16 2 6例 ) ;hERR1以剂量依赖方式促进芳香族化酶启动子Ⅰ 3活性。结论　hERR1在体内可能起到促进乳腺癌生长的作用。 Objective To study the expression of hERR1 in breast cancer tissues and para carcinoma tissues, and its effects on aromatase gene expression Methods RT PCR was applied to analyze the expression of hERR1 in breast cancer tissue and para carcinoma tissue Effects of hERR1 on aromatase gene expression were studied with transient transfection and CAT activity assay Results hERR1 expressed in both normal and malignant breast tissues, with higher levels in carcinoma tissues than in corresponding peri carcinoma tissues in the most of samples examined The transient transfection assays showed that hERR1 increased CAT transcription under the control of promoter 1 3 of the human aromatase gene in a dose dependent manner Conclusion hERR1 might increase the growth rate of breast cancer in vitro
作者 陈健 陈敏 陈彬 周长保 李渝萍 张艳 曹廷兵 周度金
机构地区 第三军医大学基础医学部生物化学与分子生物学教研室
出处 《第三军医大学学报》 CAS CSCD 北大核心 2002年第10期1233-1235,共3页 Journal of Third Military Medical University
基金 国家自然科学基金资助项目 ( 30 0 70 359) "两个基地"建设专项基金资助项目 ( 9910 76 194 6 )
关键词 芳香族化酶 hERR1 乳腺癌 基因表达调控 人雌激素受体相关受体1 逆转录-聚合酶链反应 human estrogen receptor related receptor 1 breast cancer regulation of gene expression
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1Giguere V,Yang N,Segui S,et al. Identification of a new class of steroid hormone receptors[J]. Nature,1988,331(6 151):91-94.
  • 2Bonnelye E, Vanacker J M, Dittmar T,et al. The ERR-1 orphan receptor is a transcriptional activator expressed during bone development[J]. Mol Endocrinol,1997,11(7):905-916.
  • 3Sladek R, Bader J A, Giguere V. The orphan nuclear receptor estrogen-related receptorα is a transcriptional regulator of the human medium-chain acyl coenzyme A dehydrogenase gene[J]. Mol Cell Biol,1997,17(9):5 400-5 409.
  • 4Johnston S D, Liu X, Zuo F, et al. Estrogen-related receptor α1 functionally binds as a monomer to extended half-site sequences including ones contained within estrogen-response elements[J]. Mol Endocrinol,1997,11(3):342-352.
  • 5Yang N, Shigeta H, Shi H P, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promotor[J]. J Biol Chem,1996,271(10):5 795-5 804.
  • 6Zho F, Mertz R J. Simian virus 40 late gene expression is regulated by members of the steroid/thyroid hormone receptor surperfamily[J].Proc Natl Acad Sci USA,1995,92(19):8 586-8 590.
  • 7Yang C, Zhou D, Chen S. Modulation of aromatase expression in the breast tissue by ERRα-1 orphan receptor[J]. Cancer Res,1998,58(24):5 695-5 700.
  • 8Vanacker J M, Bonnelye E, Chopin Delannoy S, et al. Transcriptional activation of the nuclear receptor ERRα (estrogen receptor-related receptor-α)[J]. Mol Endocrinol,1999,13(5):764-773.
  • 9Zhou C, Zhou D, Esteban J, et al. Aromatase gene expression and its exon usage in human breast tumors.Detection of aromatase messenger RNA by reverse transcription-polymerase chain reaction (RT-PCR). J Steroid Biochem[J]. Mol Biol,1996,59(2):163-171.

同被引文献27

  • 1陈健,陈敏,陈彬,李渝萍,李强,周度金.视黄醇结合蛋白RBP4可与多种核受体相互作用[J].中国生物化学与分子生物学报,2004,20(5):696-701. 被引量：9
  • 2李渝萍,陈敏,陈彬,陈健,李强,周度金.肌钙蛋白I2的原核表达及与ERRα1的体外相互作用[J].中国生物化学与分子生物学报,2005,21(5):656-660. 被引量：3
  • 3陈彬 陈敏 等.核受体辅调节子结构与功能[J].第三军医大学学报,2001,23(1):149-151.
  • 4J萨姆布鲁克 E F 弗里奇 T 曼尼阿蒂斯著. 金冬雁 黎孟枫译.分子克隆实验指南:第2版[M].北京:科学出版社,1998.34-68.
  • 5Giguére V, Yang N, Segui P, Evans R M. Identification of a new class of steroid hormone receptors. Nature, 1988, 331(6151) :91 ～ 94.
  • 6Yang C, Zhou D J, Chen S. Modulation of aromatase expression in the breast tissue by ERR alpha-1 orphan receptor. Cancer Res, 1998,58(24) :5695 ～ 5700.
  • 7Johnston SD, Liu X, Zuo F, Eisenbraun T L, Wiley S R, Kraus R J,Mertz J E. Estrogen-related receptor alpha 1 functionally binds as a monomer to extended half-site sequences including ones contained within estrogen-response elements. Mol Endocrinol, 1997,11 (3): 342 ～ 352.
  • 8Zhou D, Quach K M, Yang C. PNRC: a proline-rich nuclear receptor coregulatory protein that modulates transcriptional activation of multiple nuclear receptors including orphan receptors SF1 (steroidogenic factor 1) and ERRalphal (estrogen related receptor alpha-1 ). Mol Endocrinol, 2000,14 (7) :986 ～ 998.
  • 9Zhou D, Chen S. PNBC2 is a 16 kDa coactivator that interacts with nuclear receptors through an SH3-binding motif. Nucleic Acids Res,2001,29 (19) :3939 ～ 3948.
  • 10Hong H, Yang L, Stallcup M R. Hormone-independent transcriptional activation and coactivator binding by novel orphan receptor ERR3. J Biol Chem, 1999, 274 (32): 22618 ～ 22626.

引证文献4

二级引证文献9

第三军医大学学报
2002年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部