摘要
目的 探讨一氧化氮对垂体前叶黄体生成素 (LH)及卵巢雌二醇 (E2 )释放的调节作用。方法 在大鼠垂体组织培养液及卵巢组织培养液中分别加入不同浓度的一氧化氮生成抑制剂N 硝基 L 精氨酸 (NNLA)或N 硝基 L精氨酸甲酯 (NAME)和一氧化氮供体硝普钠 (SNP) ,孵育 6 0min ,收集培养液 ,用放射免疫法测定其中LH、E2 含量。结果 NNLA增加垂体组织培养液中的LH含量 ,NAME可增加卵巢组织培养液中的E2 的含量 ,而SNP则使LH及E2 含量减少 ,这些作用具有剂量依赖性。当NNLA或NAME的浓度为 5× 10 -3 mol·L-1时 ,LH及E2 含量分别增加至基础水平的 5 .5倍及 2 .0倍 ,而当SNP浓度为 5× 10 -3 mol·L-1时 ,LH及E2 含量则分别减至基础水平的 5 0 %及 36 %。结论 一氧化氮抑制垂体前叶LH及卵巢E2 的释放 ,可能在垂体及卵巢的功能调节中起重要作用。
Objective To study the regulation of nitric oxide on the release of luteining hormone (LH) from the pipuitary and estradiol (E 2) from the ovary. Methods Nitric oxide synthase inhibitors, N G-nitro-L-arginine(NNLA) and N G-nitro-L-arginine methyl ester (NAME), and nitric oxide donor, sodium nitroprusside (SNP) at various concentrations were, respectively, added into the culture solutions, in which the rat pipuitary and ovary tissues were cultured. After 60 mins, culture solutions were collected, and the LH and E 2 contents were determined by radioimmunoassay.Results NNLA and NAME increased, and SNP decreased LH and E 2 contents in the culture solutions, respectively, in a dose-dependent manner. LH and E 2 contents were, respectively, increased by 5.5 and 2.0 times, and decreased by 50% and 36% over the base level at concentration of 5×10 -3 mol·L -1 of the agents used. Conclusion Nitric oxide inhibits LH release from the anterior pipuitary and E 2 from the ovary, and may play an important role in regulating pipuitary and ovary functions.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2002年第5期435-437,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
一氧化氮
大鼠
垂体黄体生成素
卵巢
雌二醇释放
nitric oxide
luteining hormone
estradiol
tissue culture
pipuitary
ovary
rat
