摘要
本文报道对禽网状内皮组织增殖病病毒(REV)进行的一些研究,电镜观察表明REV是典型的RNA肿瘤C型病毒,具有“苯环状结构”,动物感染试验表明REV与火鸡疱疹病毒(HVT)之间可产生协同作用来危害鸡群。作者采用了冰冻切片结合荧光抗体或酶标抗体技术来检测组织中的REV抗原,采用间接荧光抗体试验对广东10个鸡场进行了血清学调查,结果表明只有2个鸡场是REV抗体阴性的,其余8个场均呈不同程度的抗体阳性,特别值得提到的是,其中1个场两批鸡因注射不同来源的HVT疫苗而表现出REV抗体阳性率的显著差异,这显示了“HVT疫苗失效”一个很可能的原因是REV污染了HVT疫苗。
This paper reports studies on the morphology, animal infection, serological diagnosis and epidemiological survey of REV. Electron microscopy showed that REV was a typical type C oncornavirus with a 'benzene ring-like' structure. Animal infection demonstrated that there existed synergism between REV and Herpesvirus of Turkey (HVT), and their combined action was dangerous to chicks. Using fluorescent antibody and enzyme-linked antibody techniques on frozen sections, two methods were established for determining REV distribution in the body of infected chicks. Serological survey of 10 chicken farms in Guangdong Province revealed positive reactors in 8 out of 10 farms, and suggested that the possible reason for HVT vaccine failure at one chicken farm was that the HVT vaccine used had been contaminated with REV.
出处
《华南农业大学学报》
CAS
CSCD
1991年第A11期69-74,共6页
Journal of South China Agricultural University
关键词
鸡
免疫
网状内皮增殖
病毒
分布
REV
HVT
Synergism
Immunosuppression
Virus distribution
