摘要
本研究以秋水仙素为微管破坏剂,注入雄性大鼠体内,在光镜和透射电镜下观察睾丸Sertoli细胞及生精上皮的形态学变化,以探讨微管对Sertoli细胞形态及生精上皮结构的作用。注入秋水仙素后,Sertoli细胞突起回缩,外形显著改变,细胞内微管消失,线粒体及内质网等分布紊乱。生精上皮中出现许多裂隙,细胞排列不整齐,深入生精上皮层内的长形精子细胞移至上皮表面。生精上皮基底层形态变化不显著。上述结果进一步验证,Sertoli细胞内微管对细胞整体形态的维持、细胞内亚细胞结构的分布、生精上皮细胞的排列及长形精子细胞的移动均有重要作用。
To investigate the function of microtubules(MTs)in Sertoli cell,colchicine,a microtubule disrupting agents,was injected into the dorsum of the male SD rat.The seminiferous tubules and Sertoli cell of the rat were examined by light and electron microsopy.5-6 hours after the colchicine injection the following results were revealed(1)The Sertoli cell of the rat testis showed prominent morphological changes.The processes of the cell retracted,the MTs disappeared in the cytoplasm and the distribution of mitochondria and smooth endoplasmic reticulum became irregular.(2)Many widened intercellulor spaces occured in the seminiferous epithelium;and accordingly the arrangement of the germ cells became deranged and the elongated spermatids moved form their original deep position to the surface of the epithelium.(3)There was no evident changes in the basal region of the seminiferous epithilium.These results confirmed that MTs in Sertoli cell are essential to the maintenance of the normal shape and cellular organelle arrangement in the cell as well as the architecture of seminiferous epithelium.They may also play an important role in the movement of the elongated spermatids.
作者
朱立基
佟大山
宗书东
Zhu Liji;Tung Dashan;Zong Shudong(Department of Anatomy,Peking Union Medical College,Beijing;Department of Cell Biology,National Research Institute for Family Planning,Beijing)
出处
《解剖学报》
CAS
CSCD
北大核心
1991年第3期292-296,T010,共6页
Acta Anatomica Sinica
基金
国家"七五"课题资助项目
