摘要
目的 观察乳腺癌组织中NOEY2基因的mRNA表达情况 ,并探讨其与临床病理及其他分子指标的关系。方法 以RT PCR法和反义RNA探针原位杂交法 ,检测乳腺良恶性病变组织中NOEY2基因的mRNA表达。以免疫组化法检测乳腺癌石蜡标本中的雌激素受体 (ER)状态、Ki6 7标记指数以及p2 7和p2 1WAF1的表达。结果 RT PCR法检测显示 ,2 4例冻存乳腺组织中 ,6例良性病变NOEY2均为阳性 ;18例乳腺癌中 ,13例 (72 .2 % )NOEY2为阳性。原位杂交法检测显示 ,10例乳腺良性病变NOEY2均为阳性 ;而 6 0例乳腺侵袭性癌中 ,仅有 31例NOEY2为阳性 ,占 5 1.7%。良恶性病变NOEY2阳性率差异有显著性 (P <0 .0 2 5 )。NOEY2的阳性率有随组织学分级升高而递减的趋势。淋巴结阴性者阳性率为 76 .7% ,而阳性者仅为 2 6 .7% ,两者差异有显著性 (P <0 .0 0 1)。NOEY2基因mRNA表达与其他临床病理指标以及免疫组化指标均无显著相关性。结论 NOEY2基因可能在一定程度上参与了乳腺癌的发生和发展 。
Objective To investigate the expression of NOEY2 gene in breast cancer tissue and its relation to clinicopathological and other molecular parameters. Methods The mRNA expression of NOEY2 gene was monitored in benign and malignant breast lesions by RT PCR and in situ hybridization (ISH) with transcripted antisense RNA probes. The protein expression of estrogen receptor (ER), Ki67, p27 and p21 WAF1 in the 60 breast cancer lesions was detected by immunohistochemical method. Results All 6 benign lesions, and 13 (72.2%) of the 18 breast cancers were NOEY2 positive by RT PCR. By ISH, positive NOEY2 was obtained in all 10 benign lesions but only in 31(51.7%) of the 60 breast cancer lesions. The difference was statistically significant ( P = 0.025 ). NOEY2 positive rate tended to decrease with the increase of histological grade. However, NOEY2 expression was negatively correlated with the status of axillary lymph nodes. The positive NOEY2 rate was 75% in those without lymph node metastasis but only 26.7% in those with metastasis ( P < 0.001 ). No correlation with other clinicopathological parameters including ER, Ki67, p27 or p21 WAF1 were found. Conclusion NOEY2 gene may be related with the pathogenesis of breast cancer. A link between NOEY2 loss expression and the spreading mechanism of breast cancer may possibly exist.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2002年第5期475-478,共4页
Chinese Journal of Oncology
