摘要
报道了长牡蛎四倍体诱导的新技术。采用长牡蛎二倍体卵子与精子授精 ,用 1.5mg/dm3 CB在受精后4min处理受精卵 40min抑制第一和第二极体排放诱导四倍体 ,获得稚贝 (1～ 3mm) 2 0 0 0粒 ,其中四倍体占85 %,三倍体占 5 %,三倍体 /四倍体嵌合体占 10 %。本文首次报道了可存活的三倍体 /四倍体嵌合体稚贝。
The new technology of induction tetraploid is reported. Tetraploid was induced by inhibiting polar body I and Ⅱ in eggs from dioploid Crassostrea gigas fertilized with haploid sperms at concentration of 1.5mg/dm 3 CB for 40min at 4 min after fertilisation, and 2000 spats were obtained. The tetraploid was 85%, the triploid was 5% and the triploid/tetraploid mosaics was 10%. This is the first report about the survival triploid/tetraploid mosaics spats.
出处
《上海水产大学学报》
CSCD
2002年第3期248-252,共5页
Journal of Shanghai Fisheries University
