摘要
根据酶标免疫法原理 ,用含有针对兔IgG(己烯雌酚抗体 )的羊抗体与待测中华鳖肌肉中的己烯雌酚发生特异性的结合反应。洗涤后 ,加入的酶标底物连接未结合的己烯雌酚抗体 ,在酶基质的作用下与无色发色剂生成有色产物。反应后于 45 0nm处测量吸光度 ,吸光强度与样品中的己烯雌酚浓度成反比。该检测方法平均最低检测下限为 0 .0 12 5 μg/kg ,平均回收率 74.8%,变异系数为 1.76 3～ 3.0 84%。表明本方法快速、灵敏、可靠 ,适合对己烯雌酚残留限量检测的要求。
Based on the antigen-antibody reaction, diethylstilbestrol (DES) in the muscle of Trionyx sinensis is bound by the sheep antibodies that are directed against rabbit IgG (anti DES antibody) in the reagent box. After a washing step the DES enzyme conjugate is added, which is bound by the remaining free antibody binding sites. With the enzyme substrate, the colorless chromogen converts into a blue product. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the DES concentration in the sample. The mean lower detection limit of this method is about 0.0125μg/kg. The mean recovery rate is 74.8% and the coefficients of variation is 1.763～3.084%. This method is rapid, sensitive and reliable. It is available for sensitive detection of DES residues.
出处
《上海水产大学学报》
CSCD
2002年第3期199-202,共4页
Journal of Shanghai Fisheries University
