摘要
为研究洛阳市汉族群体ADH2和ALDH2基因的多态性分布,应用聚合酶链反应-扩增片段长度多态性（PCR-APLP）分析法,对ADH2基因外显子3和ALDH2基因外显子12的特定片段同时进行特异性扩增,用非变性的聚丙烯酰胺垂直凝胶电泳和DNA银染方法判定基因型。ADH2＊1和ADH2＊2等位基因频率分别为42.86％和57.14％,ADH2＊1/＊1、＊1/＊2和＊2/＊2的基因型频率分别为22.86％、40.00％和37.14％;AL-DH2＊1和ALDH2＊2的等位基因频率分别为85.24％和14.76％,ALDH2＊1/＊1、＊1/＊2和＊2/＊2的基因型频率分别为71.43％、27.62％和0.95％。洛阳市汉族群体ADH2和ALDH2的等位基因频率和基因型频率不同于台湾人和上海人,ALDH2＊1/＊1基因型频率明显高于上海人和台湾人的。因而,洛阳市居民对酒精的耐受性比上海人和台湾人强。
In order to investigate genetic polymorphisms of ADH2 and ALDH2 among the Han population in Luoyang City, portions of exon 3 of ADH2 and exon 12 of ALDH gene were amplified by using polymerase chain reaction. The amplified products were electrophoresed on 10% undenatured vertical polyacrylamide gels and stained with argentine. Frequencies of ADH2 * 1 and ADH2 * 2 alleles are 42. 86% and 57. 14%. Frequencies of three genotypes of ADH2 are 22. 86% ,40. 00% and 37. 14% .respectively. Frequencies of ALDH2 * 1 and ALDH2 * 2 alleles are 85.24% and 14. 76%. Genotype frequencies of ALDH2 loci are 71. 43% N27. 62% and 0. 95% respectively. Genetic polymorphisms of ADH2 and ALDH2 among the Han population in Luoyang City are different from those among Taiwan Residents and Shanghainese. Frequency of ALDH2 * 1 / * 1 in Luoyang people is higher than those in Shanghai and Taiwan. Therefore, there is a higher resistance to alcohol drinking in the Han population in Luoyang.
出处
《遗传》
CAS
CSCD
北大核心
2002年第5期532-536,共5页
Hereditas（Beijing)
基金
国家自然科学基金(编号:39870654)资助
关键词
洛阳市
汉族群体
ADH2
ALDH2
基因多态性
乙醇脱氢酶2
乙醛脱氢酶
polymerase chain reaction-amplified products length polymorphism
alcohol dehydrogenase 2
aldehyde dehydrogenase 2
genetic
