摘要
葡萄球菌B型肠毒素(SEB)是重要的超抗原,依据SEB分子的晶体结构并结合表面分析,模拟设计了与MHCⅡ类分子和TCRVβ区亲合力降低的三位点突变体mSEB(Y89A,C93S,Y94A)。通过PCR重叠延伸法获得突变体基因,导入PBV220载体中,于E.coliDH5α中获得高效表达,纯化的突变毒素T细胞激活活性仅为野生型毒素的1/5左右。
Mutant staphylococcal enterotoxin B,which was suggested to have a low affinity to both MHC-II complex and T Cell receptor,was designed by3-D structure and surface property analysis.The mutant gene was constructed by PCR over-extension method and inserted to prokaryotic expression vector PBV220for a high expression in E.coli system.Moreover,the purified mutated products showed only1/5T cell stimulation potentency of the wild-type Staphylococcal enterotoxin B.
出处
《生物技术通讯》
CAS
2002年第5期341-343,共3页
Letters in Biotechnology
