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MPTP对小鼠不同脑区神经递质代谢酶基因表达的影响 被引量：1

GENE EXPRESSIONS OF NEUROTRANSMITTER METABOLISM RELATED ENZYMES IN SUBSTANTIA NIGRA AND STRIATUM OF MPTP-TREATED MICE
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摘要 1-甲基 -4 -苯基 -1,2 ,3 ,6-四氢吡啶 (MPTP)能够诱发黑质纹状体系统多巴胺能神经元损伤并产生与帕金森病类似的症状。本实验通过 MPTP注射诱导 C5 7BL小鼠神经元损伤 ,利用逆转录 PCR方法检测酪氨酸羟化酶 (TH)、单胺氧化酶 B(MAO-B)以及胆碱乙酰基转移酶 (Ch AT)三种神经递质代谢相关基因的表达变化。结果发现 ,在小鼠黑质与纹状体中 ,MPTP导致 TH基因表达显著降低 ,而 MAO-B与 Ch AT基因的表达变化在黑质与纹状体则有所不同 ,在黑质 MPTP导致 MAO-B与 Ch AT基因表达的上升 ,在纹状体则略有下降。 Administration of 1 methyl 4 phenyl 1,2,3,6 tetrahydropyridine (MPTP) to mammals causes damage to the nigrostriatal dopaminergic pathway similar to that observed in Parkinson's disease. The gene expressions of tyrosine hydroxylase (TH), monoamine oxidase (MAO B) and choline acetyltransferase (ChAT) in substantia nigra and striatum of MPTP treated C57BL mice were detected by reverse transcriptional polymerase chain reaction (RT PCR). It was found that the expression of TH gene was decreased in the substantia nigra and striatum of MPTP treated mice, the expressions of MAO B and ChAT genes were increased in substantia nigra and showed no significant difference in striatum.
作者 徐磊 刘彧 曾盈 吕立夏 李学礼 王尧
机构地区 同济大学医学院生物化学教研室
出处 《神经解剖学杂志》 CAS CSCD 北大核心 2002年第3期203-205,共3页 Chinese Journal of Neuroanatomy
关键词 MPTP 小鼠 脑区 神经递质代谢酶 基因表达 酷氨酸羟化酶 单胺氧化酶B 胆碱乙酰基转移酶 黑质 纹状体 MPTP, tyrosine hydroxylase, monoamine oxidase B, choline acetyltransferase, substantia nigra, striatum, mice
分类号 R341 [医药卫生—基础医学]
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参考文献6

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同被引文献9

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神经解剖学杂志
2002年 第3期

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