摘要
本试验培养底物及瘤胃液供体动物日粮为玉米 -豆粕型日粮 ,实验用瘤胃液来自3只安装永久性瘤胃瘘管的绵羊 ,体外实验按不同烟酸添加剂量分为13个试验组 (0、50、150、250、350、400、450、500、600、700、800、900、1000mg/kg) ,每个试验组4个重复。在体外培养24小时后 ,测定培养液中NH3-N、瘤胃细菌N产量、原虫数量、总VFA产量及乙酸、丙酸、丁酸产量和乙酸/丙酸值 ,用以确定在该氮源日粮条件下 ,添加烟酸对瘤胃发酵的影响 ,并对烟酸适宜添加范围进行了研究。实验结果表明 ,在豆粕型日粮来源培养底物中 ,烟酸适宜添加范围为450～600mg/kg。
In this experiment,three sheep fitted with permanent cannule in the rumen and fed a corn-soybean meal based diet were used to investigate the effects of niacin supplementation on rumen fermentation,and further to determine the supplemental level of niacin by in vitro technique.The substrate for incubation was mixture of concentrates and hays according the diet formula.There were thirteen treatments based on different supplemental doses of niacin.Each treatment had four duplications,the time of incubation was 24h.Parameters for observation included of NH3-N concentration,bacterial N?protozoa number?total VFA?acetate?propionate?butyrate production and acetate to propionate ratio.According to the effects of niacin supplementation on rumen fermentation,the optimum range of supplemention was 450～600mg/kg.
出处
《动物营养学报》
CAS
CSCD
2002年第3期60-64,共5页
CHINESE JOURNAL OF ANIMAL NUTRITION
