摘要
目的 探讨成纤维细胞生长因子 (bFGF)对骨髓基质细胞 (NASP)的增殖及分化作用。方法 分离骨髓基质细胞体外培养 ,化学染色后行图象分析测定细胞克隆数 ;采用免疫组化 (ABC法 )检测PCNA ,Ⅰ型胶原 ,研究bFGF对NASP的促增殖及分化作用。结果 骨髓细胞中许多基质前体细胞以非贴壁细胞形式存在 ,bFGF不仅能促进NASP细胞增殖 ,而且能诱导NASP细胞向成骨细胞分化。结论 bFGF主要作用于非贴壁生长的基质前体细胞 ;bFGF能增加骨细胞的数量 ,促进骨样组织形成。
Objective To investigate the actions of basic fibroblas growth factor(bFGF) on the proliferation and differentiation of the Non adherent stromal precursor(NASP) cells.Methods The osteogenic potential of the bone marrow stromal cells (BMSC)isolated from Wistar rats were cultured in the absence or presence of bFGF. After cytochemistry performed,the colonies were counted by image analysis;The cells cultured in petri dishes were stained by the avidin biotin peroxidase comples(ABC) immunoperoxidase technique for collagen type Ⅰ?proliferating cell nuclear antigen(PCNA).Results Many stromal precursor cells present in bone marrow in a non adherent form.bFGF not only stimulated the proliferation of NASP cells into osteoblasts.Conclusion NASP cells are possible main target of anabolic action of bFGF and it may play an important role in fracture healing and bone formation.
出处
《中国血液流变学杂志》
CAS
2002年第3期181-184,共4页
Chinese Journal of Hemorheology
