摘要
目的 探讨淀粉样前体蛋白 (APP)基因瑞典型突变 (APPSW)在Alzheimer病 (AD)发病中的作用。方法 对PDAPSW转基因小鼠的毛发、体重及生殖能力进行观察 ;采用免疫组化方法对转基因小鼠大脑组织进行病理改变分析 ;采用国际通用的Y迷宫分析转基因小鼠的行为学异常。结果 3个月龄时首建鼠平均体重为 (31.0± 3 .7)g ,非转基因小鼠为 (34.0± 2 .9)g ,F1代转基因小鼠平均体重为 (32 .0± 3 .3)g ,阴性同胞鼠为 (31.0± 4.2 )g ,两组体重差异无显著意义 ;两组转基因小鼠毛发及生殖能力差异均无显著意义 ,但发现其中 3只阳性小鼠毛发异常 ,1只雄性小鼠不育 ;转基因小鼠脑组织免疫组化分析发现明显淀粉样蛋白沉积 ;Y迷宫内转基因小鼠进入迷宫各臂均数为 (5 3± 7)次 ,非转基因鼠为 (37± 4)次 ;轮回转向频率分别为 48.2 %和 76 .4% ,两组间差异有显著意义。结论 PDAPSW转基因小鼠脑组织内有淀粉样蛋白沉积并且存在行为学异常 ,能够复制人类AD的主要变化 ,可以作为深入研究AD发病机制的动物模型。
Objective In order to study the role of APP SW mutation in the aetiology of Alzheimer′s disease(AD), the phenotypes of the PDAP SW transgenic mice were investigated. Methods Body weight, coat color and reproducibility of transgenic mice were observed;The pathological changes in the brain of the transgenic mice were examined using immunohistochemistry; Behavioral changes of transgenic mice were examined by Y-maze. Result At 3 month′s age, the mean body weight of the transgenic mice was (31.0±3.7) g, that of non-transgenic was (34.0±2.9) g;while the mean body weight of F1 transgenic mice was (32.0±3.3) g, of negative mice was (31.0±4.2) g. There were no significant difference between these two groups. There were three transgenic mice with abnormal coat and one male was infertile. The transgenic mice had amyloid deposit in their brains. In the Y-maze test, transgenic mice showed an increased number of arm entered (53±7 versus 37±4) and an impaired spontaneous alternation. The frequency of alternation was 48.2% in the transgenic mice and 76.4% in the non-transgenic mice,respectively.Conclusion The PDAP SW transgenic mice had typical pathological and behavioral changes similar to that of AD and could be used as an animal model for studying AD.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2002年第4期201-203,共3页
Chinese Journal of Neurology
基金
国家自然科学基金资助项目 ( 30 0 30 0 70 )
