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化妆品卫生微生物检验方法探讨 被引量:13

Study on Microbial Detecting Methods for Cosmetics
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摘要 目的研究金黄色葡萄球菌在化妆品中的存活能力 ,探讨不同检测方法对粪大肠菌群检测的敏感性。方法应用《化妆品卫生规范》规定的检验方法及改进的在乳糖胆盐培养基中加入中和剂的方法分别进行检测。结果化妆品中金黄色葡萄球菌随存放时间的延长 ,菌量逐渐减少 ,且在含与不含防腐剂的化妆品间存在差别 ;改进的检测粪大肠菌群的方法比国标法更敏感。结论化妆品中一些成分特别是防腐剂对金黄色葡萄球菌的存活有影响 ,在乳糖胆盐培养基中加入卵磷脂。 Objective To study the viability of S.aureus in common cosmetics and the sensitivities of different detection methods adopted in detecting faecal coliform. Methods A standard method stated in 'Hygienic Standard for Cosmetics' and lactose-bile salt medium improved by adding neutralizers were adopted in detecting Faecal coliform. Results The amounts of S.aureus dropped gradually as the prolongation of preservation period and in cosmetics containing or not containing antiseptics were different too. In detecting Faecal coliform, the sensitivity of the improved method was higher than that of the standard method. Conclusion Some ingredients of common cosmetics, especially antiseptics, obviously affected the viability of S.aureus. The lactose-bile salt broths containing Lecithin and Tween 80 were much better than those not containing Lecithin and Tween 80 in detecting faecal coliform.
出处 《环境与健康杂志》 CAS CSCD 北大核心 2002年第5期397-398,共2页 Journal of Environment and Health
关键词 化妆品 微生物敏感性试验 金黄色球菌 粪大肠菌群 存活期 Cosmetics Microbiological sensitivity test S.aureus Faecal coliform Viable period
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