摘要
采用PCR技术扩增了栉孔扇贝 (Chlamysfarreri)线粒体DNA的 1 6SrRNA基因片段 ,PCR产物经T载体连接之后进行了克隆、测序 ,得到 634bp的核苷酸序列 ;分析了该片段的大连、烟台、青岛、朝鲜 4个自然群体 31个个体的核苷酸序列多态性 ,共检测到 2 6个多态性核苷酸位点、1 8种单倍型。结果表明 ,4个群体中以朝鲜群体遗传多样性最高 ,其次为烟台、青岛群体 ,大连群体最低 ;
DNA sequencing is very useful for the genetic diversity analysis at molecular level. Mitochondrial 16S rRNA gene fragment of Scallop Chlamys farreri was amplified via polymerase chain reaction (PCR), the PCR products were ligated into T vector, cloned and sequenced for 31 individuals, 3 populations of China (8 for Dalian and Qingdao each, and 7 for Yantai) and one population of Republic of Korea. 634 base pair nucleotide sequences were examined and analyzed for genetic polymorphism. Resultant sequences data analysis showed that all 31 sequences were grouped into 18 haplotypes and there were 26 variable nucleotide positions in this gene fragment, which included transitions and transvertions, and no insertion/deletion were observed. The genetic diversity indexes including S (polymorphic sites), P (nucleotide diversity) and K (average number of nucleotide difference) were calculated and the results indicated that Korea population showed more variation than others and more differences to others, followed by Yantai and Qigndao population, and Dalian population had the least sequence variation. Parameters of genetic variation among the four populations suggested that no significant genetic difference was observed. Our data provided baseline information at DNA level on the genetic variation in wild Scallop populations and may be useful for future management and utilization of the resource.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2002年第5期477-483,共7页
Oceanologia Et Limnologia Sinica
基金
国家重点基础研究发展规划项目 (973 )资助G1 9990 1 2 0 0 8号
国家自然科学基金资助项目 3 960 0 1 1 3号
