摘要
探讨了银杏细胞悬浮培养过程中诸多因子对细胞生长率和黄酮类化合物含量的影响 ,结果表明 :液体培养基以N6 +NAA(1.6～ 1.8) +BA0 .5为最好 ;最适接种量为 2 .5g～ 3.0g 瓶 (鲜重 ) ,最适pH值为 6 .0～ 6 .5 ,附加MES效果更好 ;碳源以 4 0 %葡萄糖进行细胞培养和 30 %蔗糖进行黄酮生产较好 ;细胞生长和生产黄酮的激素、碳源等培养条件不同 ,激素类以NAA(1.6～ 1.8) +BA 0 .5进行细胞培养、以GA0 .5 +KT1.0进行黄酮生产最佳 ;附加黄酮前体物PLA可提高黄酮产量 ,放线菌素 -D不适合黄酮类药用物质生产 .
Cell suspension culture were established from Ginkgo biloba L. callus cultures. A number of factors affecting the cell growth and the flavonoid production were studied, The result showed that the optimum medium, rotation speeds of the gyratory shaker, inoculum sizes, pH values and the combination of hormone in flavonoid production were N6+ NAA(1.6～1.8)+BA0.5, 90～100 r/min rotation speeds, 2.5～3.0 g /bottle (FW) , pH 6.0～6.5 and supplament of MES respectively; The optimum concentration of 40% glucose is preferable for cell growth and 30% sucrose for flavonoid production. Adding Phenylalanine to medium, flavonoid production improved. Actinomycine D was not suitable for flavonoid production.
出处
《河南大学学报（自然科学版）》
CAS
2002年第3期20-24,共5页
Journal of Henan University:Natural Science
基金
河南省科委自然科学基金项目 (994 0 2 6 6 0 0 )
河南省教委自然科学基金项目 (974 75 0 19)
