摘要
目的 了解胰腺癌患者体内自身抗体阳性率、细胞内定位及荧光模式特点,初步确定自身抗体靶抗原,分析自身抗体与胰腺癌的相关性,探索自身抗体作为肿瘤标志物的可能性。方法 应用间接免疫荧光法(indirected immunofluorescence,IIF)检测55份胰腺癌以及60份不同年龄段正常人血清中自身抗体。提取人喉癌上皮细胞(Hep-2)蛋白抗原,采用蛋白质印迹法,对55份胰腺癌患者及52份正常人血清进行分析。结果 55份经IIF检查的胰腺癌患者中,32份(58.18％)自身抗体阳性,显著高于正常对照组26.67％(l6/60)的检出率(P<O.01),两者在荧光模式、靶抗原细胞内定位上均有显著差别。蛋白质印迹法中51份(92.73％)胰腺癌患者血清中检测到阳性条带,显著高于对照组(31/52,59.62％),其中大多数与自身免疫性疾病中常见的自身抗体不同,并发现63.64％(35/55)的胰腺癌患者具有47kDa的靶抗原的阳性共带,该条带仅在1.9％(1/52)的正常人中查及。结论 胰腺癌患者中的自身抗体谱与正常人生理性自身抗体谱和自身免疫性疾病中的自身抗体谱不同,进一步明确其47kDa的靶抗原本质及其生物学和临床意义,可能有助于深入认识胰腺癌中的自身免疫现象,并有可能应用于胰腺癌的诊断。
Objective To study the positive rate of autoantibodies in pancreatic cancer patients, the fluorescence pattern and the location of target antigens in cells, to identify essence of the target antigens, and to explore the possible relationship between pancreatic cancer and the autoantibodies. Methods Using indirected immunofluorescence (IIF), autoantibodies in sera of 55 pancreatic cancer patients and 60 healthy controls were detected. Using antigens extracted from human epithelial cell line (Hep-2), sera of 55 pancreatic cancer patients and 52 healthy controls were analyzed with Western blotting. Results The positive rate of autoantibodies in pancreatic cancer patients was significantly higher than that in control group (58. 18% versus 26. 67%). The fluorescence pattern and the location of target antigens in cells were quite different between the two groups. Western blotting showed that one or more positive bands were detected in 51 of the 55 pancreatic cancer patients(92. 73%), which is significantly higher than that in control group(31/52,59. 62%)(P<0. 01). Most of these autoantibodies were not explainable by the common autoantibodies known in autoimmune diseases. A common positive band with related molecular weight about 47kDa was observed in 35 of the 55 patients(63. 64%), while in one of the 52 controls(1. 9%). Conclusions Autoantibodies in pancreatic cancer are different from those in normal people. Identification of the target antigen of the 47kDa common autoantibody may help understand its biological role in pancreatic cancer, and possibly be used in the diagnosis of pancreatic
出处
《胰腺病学》
2002年第3期147-150,共4页
Chinese JOurnal of Pancreatology
基金
卫生系统百名跨世纪优秀学科带头人培养计划资助基金[沪卫科(1999)21号]
关键词
自身抗体
胰腺癌
相关性
检测
Pancreatic cancer
Autoantigen
Autoantibody
