摘要
目的 :观察清热解毒、活血化瘀、养阴增液 3种温病治法制剂对内毒素血症大鼠生存率的影响。方法 :将健康 Wistar大鼠随机分为正常对照组、模型对照组及清热解毒组、活血化瘀组和养阴增液组。模型对照组和3种中药干预组大鼠腹腔注射内毒素和 D氨基半乳糖以建立内毒素血症模型 ;记录 2 4小时内各组大鼠死亡数。各组存活大鼠中随机选取 10只检测其血液流变学、肝酶谱及细胞因子的变化。结果 :模型对照组大鼠生存率达 5 5 %,清热解毒组、活血化瘀组和养阴增液组大鼠生存率明显升高 (分别为 90 %、95 %和 95 %)。模型对照组大鼠肿瘤坏死因子 α(TNFα)、白介素 1(IL 1)及天冬氨酸转氨酶 (AST)、丙氨酸转氨酶 (AL T)、碱性磷酸酶 (AL P)明显高于正常对照组 (P均 <0 .0 5 ) ;而各中药干预组上述诸指标均显著低于模型对照组(P<0 .0 5或 P<0 .0 1) ,部分指标乃至接近正常水平。模型对照组和 3种中药干预组间血液流变学各项指标均无明显差异 (P均 >0 .0 5 )。结论 :3种温病治法制剂——清热解毒、活血化瘀和养阴增液注射液均可显著提高内毒素血症大鼠生存率 ,拮抗炎性细胞因子 ,减轻肝细胞损伤 ,对内毒素血症大鼠有一定的保护作用。
Objective:To explore and analyze the effect of three antifebrile herbal preparations 〔clearing heat and detoxification (清热解毒),activating blood and removing stasis (活血化瘀),supplementing Yin and increasing fluids (养阴增液)〕 on the survival rate of endotoxemic rats.Methods:The healthy Wistar rats were randomly divided into the normal control group,model control group,clearing heat and detoxification group, activating blood and removing stasis group and supplementing Yin and increasing fluids group.The murine model of endotoxemia was established by the intraperitoneal injection of lipopolysaccharides and dgalaclosamine in model control group and three treated groups.The dead rats in each group at 24 hours after endotoxin infusion were accounted.The blood rheology parameters,serum spectrum of liver enzymes and cytokines levels were determined in the survival rats of each group.Results:The survival rate in model control group was down to 55%,while the survival rates in clearing heat and detoxification group,activating blood and removing stasis group,supplementing Yin and increasing fluids group were greatly elevated (90%,95% and 95% respectively).The serum levels of tumor necrosis factorα(TNFα) and interleukin1(IL1) and the activities of aspartate aminotransferase (AST),alanine aminotransferase (ALT),alkaline phosphatase (ALP) in model control group were significantly higher than those in normal control group (all P<0.05),while serum cytokines levels and liver enzymes activities in three treated groups were remarkably decreased in comparison with those in model control group (P<0.05 or P<0.01).There was no significant difference of the rheology parameters between model control group and three treated groups.Conclusions:The administration of three antifebrile herbal preparations 〔clearing heat and detoxification,activating blood and removing stasis,supplementing Yin and increasing fluids〕 to treat endotoxemia would greatly elevate the survival rate of the endotoxemic rats,antagonize the inflammatory cytokines and attenuate the damage of liver cells.The three antifebrile herbal preparations would possess the protective effects to the endotoxemic rats.
出处
《中国中西医结合急救杂志》
CAS
2002年第5期253-255,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然科学基金资助项目 ( No.39870 92 5 )
关键词
温病治法制剂
内毒素血症
大鼠
生存率
研究
清热解毒
活血化瘀
养阴增液
endotoxemia
survival rate
clearing heat and detoxification
activating blood and removing stasis
supplementing Yin and increasing fluids
