摘要
建立了一种简便、灵敏的测定血浆中曲他维啉的高效液相层析方法。血浆中曲他维啉在碱性条件下被乙醚提取 ,然后用 0 .1mol/L盐酸反提取。 1 0 μL提取液进C1 8柱 ,检测波长 2 5 4nm ,流动相为 0 .0 2mol/L磷酸二氢 钠∶甲醇 =30∶70 ,高氯酸调 pH至 2 .8。曲他维啉与血浆中其他组分和内标能很好分离。本方法具有良好的线性关系 ,相对标准偏差小于 1 5 % ,检出下限 (信号噪声比 =3∶1 )为 8μg/L ,回收率 80 %～ 1 0 0 %。
A simple and sensitive HPLC method for the determination of drotaverine in human plasma has been developed. Drotaverine in alkalinized plasma was extracted with aether solvent and the drotaverine in the aether phase was back-extracted into 0.1 mol/L HCl. 10 μL of the aqueous layer was injected into the C18-column and the eluent was monitored at 254 nm. Separation was performed on the column with 0.02 mol/L sodium dihydrogen phosphate-methanol (30∶70) containing perchlorate at pH 2.8 as mobile phase. Drotaverine was well resolved from the plasma constituents and internal standard. An excellent linearity was observed between peak-height ratios and plasma concentrations and the RSD was always <15%. The lowest limit of detection (signal-to-noise ratio 3∶1) was 8 μg/L, the recovery rate was between 80%～100%. The method is suitable for therapeutic monitoring and pharmacokinetic studies of drotaverine in humans.
出处
《首都医科大学学报》
CAS
2002年第3期208-210,共3页
Journal of Capital Medical University
