摘要
目的 探讨临床药敏试验对医院感染控制的指导意义。方法 医院检验科收集临床分离菌1229株,应用K-B琼脂扩散法进行药敏试验,对306株大肠埃希菌和肺炎克雷伯菌,应用纸片法检测超广谱β-内酰胺酶(ESBLs)。结果 在革兰阴性杆菌中铜绿假单胞菌的耐药指数最高,在革兰阳性球菌中肠球菌耐药指数最高,亚安培南对革兰阴性菌抑菌效果明显优于其他抗生素,万古霉素对革兰阳性球菌的抑菌效果最佳,从大肠埃希菌,肺炎克雷伯菌中检出产ESBLs的菌分别为28.5％和30.3％,产酶菌株对头孢菌素高度耐药,对环丙沙星,庆大霉素及复方新诺明等耐药率明显高于非产酶菌(P<0.01),而对亚安培南则敏感(P<0.05)。结论 耐药指数越高,耐药面越广,耐药率越高。
[Objective] To study the instructive significance of drug sensitive test on the infection control works in hospitals.[Methods] 1229 cases of clinically isolated strain bacteria were collected and received drug sensitive test with K-B agar diffusion method.β-ESBLs was tested with paper scrap method in 306 escherichia coli and klebsiella pneumoniae.[Results] 64.0% of the infections were caused by G-bacteria and 36.0% of them were caused by G + coccus.The pseudomonas aeruginosa has the highest antibiotic resistant index to G-bacterium.The enterococcus has the highest antibiotic resistant index to G + coccus.The G-bacteria were susceptible to impenem.The G + coccus were susceptible to vancomycin.Extended spectrumβ-lactamases producers were 28.5 % and 30.3% from escherichia coli and klebsiella pneumoniae.These strains were highly resistant to the cephalosporins than that of non-producers ( P < 0.01),but susceptible to impenem ( P > 0.05).[Conclusion] The higher the drug resistant index is,the wider it is,and the higher the resistant rate is.
出处
《职业与健康》
CAS
2002年第10期38-40,共3页
Occupation and Health
关键词
细菌
药敏试验
Β-内酰胺酶
抗生素
医院感染
耐药率
Bacteria, Drug sensitive test,β- lactamases, Antibiotic, Infection in hospital, Drug resistant rate.
