摘要
目的 :对北京和东北两地西洋参种子样品的带菌量和带菌种类进行检测。方法 :分别用水洗法和表面消毒培养法分离种子表面和种子内部寄藏真菌。结果 :13个西洋参种子样品携带真菌类群主要有镰刀菌属Fusariumspp .、链格孢属Alternariaspp .、青霉属Peniciliumspp .、头孢霉属Cephalosporiumsp .、根霉属Rhizopusspp .及少量的毛霉属Mucor、曲霉属Agspergillus、木霉属Tritroderma和矛菌属Doratomyces等共十余个属的劣势真菌。结论 :西洋参种子带菌率较高 ,种壳带菌率为 36 %～ 10 0 % ,种胚带菌率为 4 0 %～ 10 0 %。不同地点的种子外部带菌量差异明显 ,种胚寄藏的优势真菌种类和分离频率与种壳的结果基本一致。
Objective: The quantity and genus of fungi on 13 samples of American ginseng seed from Beijing and Northeast area in China were detected. Method: Washing and surface sterilization were used to isolate the fungi existed on seed surface and inside of the seed, respectively. Result: The genera of the fungi in embryo and shell of the seeds mainly belonged to Fusarium spp., Alternaria spp., Penicilium spp., Cephalosporium sp., Rhizopus spp., and a small quantity of Mucor, Aspergillus, Tritroderma, Doratomyces and so on. The isolated fungal frequencies on the shell and in the embryo were 36%～100% and 40%～100% respectively. Conclusion: The fungal frequency of American ginseng seed was higher. There were significant differences among different seed samples, the main fungal genera and isolation frequency in the seed embryo were identical with the results from the seed shell.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2002年第9期658-661,共4页
China Journal of Chinese Materia Medica
基金
中药现代化研究与产业化开发项目 (99 92 9 0 3 0 9)
