摘要
206例不同病理诊断的宫颈脱落细胞和宫颈组织DNA,同探针杂交,检测HPV_(16)DNA同源性序列。结果表明:HPV_(16)DNA探针同宫颈癌脱落细胞杂交(65.2％)和同宫颈癌组织DNA杂交(66.7％),其杂交率无显著差别(P>0.05)。宫颈炎脱落细胞DNA和宫颈炎组织DNA与HPV_(16)DNA探针杂交率间的差异也无统计学意义。然而,宫颈癌和宫颈炎之间无论是脱落细胞或是组织DNA同探针杂交有显著差异(前者为65.2—66.7％,后者为26.1—28.5％)P<0.01。宫颈癌和宫颈炎DNA经酶切电泳后,Southern blot杂交结果提示HPV_(16)DNA整合在癌组织基因组中。
DNA extracted from the cervical tissues and cervical decidual, cell were detected by hybridization of nucleic acid with the probe of (α—32p)—dCTP labeled 7.9Kb HPV DNA. The positive rate of HPV16 DNA of decidual cell DNA was 65.2％, of cervical cancer tissues DNA was 66.7％ and had no significant difference, but differences between cervical cancer and cervicitis, eithcr decidual cell DNA or tissue DNA hybridized with probe all had significant difference (former 65.2—66.7％, latter 26.1—28.5％), P<0.01.
The Physical state of HPV16 DNA in cervical cancer southern blot and twe-dimentional electrophoresis were evaluated and showed that HPV16 DNAwas integrated in cervical cancer cell genome.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1991年第3期142-144,共3页
Cancer Research on Prevention and Treatment
基金
湖北省科学技术基金
关键词
子宫颈肿瘤
人乳头病毒
DNA
Cervical cancer, IntegratiOn, HPV16 DNA, Molecular hybridization.
