摘要
目的检测蛛网膜下腔出血大鼠模型脑组织中神经肽YmRNA表达的变化,为进一步研究防治蛛网膜下腔出血后脑血管痉挛提供线索。方法建立大鼠蛛网膜下腔出血模型,并设生理盐水模型和假手术模型对照,用RT-PCR方法半定量检测各模型分别存活15、30min和1、4、8、12、24、48h时纹状体、大脑皮层、下丘脑中神经肽YmRNA的含量。结果蛛网膜下腔出血后脑组织中神经肽YmRNA的含量显著升高;随时间的推移,含量逐渐下降,但仍显著高于同一时点的生理盐水组和假手术组。结论蛛网膜下腔出血后脑组织中神经肽YmRNA的含量显著升高,这可能在诱发脑血管痉挛中起重要作用。
Objective To investigate the changes in the expression of neuropeptide Y mRNA in the cerebral tissues of rat models of subarachnoid hemorrhage (SAH), to shed light on the prevention and treatment of cerebral vasospasm induced by SAH. Methods SAH rat models, saline and sham-operation control groups were respectively established, in which the relative abundance of neuropeptide Y mRNA in the corpus striatum, cerebral cortex, hypothalamus were subsequently measured and compared by reverse transcriptase PCR at 15 min, 30 min and 1, 4, 8, 12, 24, 48 h after corresponding treatment. Results The abundance of neuropeptide Y mRNA significantly elevated in the cerebral tissues after SAH, and gradually declined with the passage of time, but were still significantly higher than that of saline and sham-operation controls at any of the same time points. Conclusions Neuropeptide Y mRNA level in the cerebral tissues significantly elevates after SAH, which may play an important role in inducing cerebral vasospasm.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第9期779-781,共3页
Journal of First Military Medical University
基金
广东省医学科研基金(A1999354)
关键词
神经肽Y
蛛网膜下腔出血
脑血管痉挛
动物模型
mRNA
定量RT-PCR
SAH
CVS
neuropeptide Y
subarachnoid hemorrhage
cerebral vasospasm
animal model, rat
mRNA
quantitative reverse transcriptase polymerase chain reaction
