摘要
利用稳定的新型铕荧光络合物BHHCT与Eu3 + 标记羊抗人HBsAb和Eu3 + 标记BSA -SA ,建立定量测定血清HBsAb的Eu3 + -BHHCT -HBsAb -TRFIA法和Eu3 + -BHHCT -BSA -SA -TRFIA法。结果表明 ,这两种方法最低检出值分别为 0 .2ng/mL和 0 .0 5ng/mL ,标准曲线范围均为 0 - 10 0ng/mL ,批内变异系数CV均小于10 % ,后者回收率为 85 % - 115 %。用Eu3 + -BHHCT -BSA -SA -TRFIA法与ELISA法同时检测 118份血清样品 。
A new and stable fluorescent europium chelate Eu 3+ -BHHCT was used as a label to develop two time-resolved fluoroimmunoassays for HBsAg. One was Eu 3+ -BHHCT-HBsAb-TRFIA, another was Eu 3+ -BHHCT-BSA-SA-TRFIA. The detection limits of the two methods were 0.2ng/mL and 0.05ng/mL respectively. The standard curves for both methods were in the ranges of 0-100ng/mL and the within-run coefficient variations were less than 10%. The recovery rates of latter method were within 85%-115%. 118 patient samples were totally detected by Eu 3+ -BHHCT-BSA-SA-TRFIA and compared with a conventional ELISA, the results showed that the former could detect more positive samples than the latter.
出处
《标记免疫分析与临床》
CAS
2002年第3期157-160,共4页
Labeled Immunoassays and Clinical Medicine
