摘要
目的 研究放射性核素 1 88铼 (1 88Re)诱导乳腺癌ER- 75 - 30细胞凋亡及其与 bcl- 2和 bax基因表达的关系 .方法 应用光镜、电镜、流式细胞仪和免疫组化方法检测不同浓度 1 88Re作用于体外培养的乳腺癌 ER- 75 - 30细胞后 ,诱导细胞凋亡情况及 bcl- 2和 bax基因表达情况 .结果 1 88Re能诱导乳腺癌 ER- 75 - 30细胞发生凋亡形态学变化 ,并且随着 1 88Re浓度增大 ,凋亡率增加 ,bcl- 2表达减弱 ,bax表达增强 .结论 1 88Re能诱导乳腺癌 ER- 75 - 30细胞凋亡且具有剂量和周期依赖性 ,bcl- 2和 bax基因在 1 88Re诱导的细胞凋亡过程中具有重要作用 .
AIM To study apoptosis of human breast cancer ER 75 30 cell induced by 188 Re and expression of bcl 2 gene and bax gene. METHODS Light microscope, transmissional electron microscope, flow cytometer and immunohistochemical method were used to observed ER 75 30 cells apoptosis after expose to 188 Re of different doses and expressing of bcl 2 and bax. RESULTS 188 Re can induced ER 75 30 cell producing typical morphologic changes of apoptosis and with the rise of radiation dose, cell apoptosis rate increased, bcl 2 gene decreased and bax gene was enhanced. Cells were blocked in G2/M period. CONCLUSION Radionuclide 188 Re can induce tumor cell apoptosis. This effect takes on dose effect relation and cellcycle dependent. bcl 2 and bax gene play import part in the course.
出处
《第四军医大学学报》
北大核心
2002年第17期1537-1540,共4页
Journal of the Fourth Military Medical University
