摘要
腹腔注射板蓝根多糖(ⅡP)50mg/kg 可显著促进小鼠免疫功能,表现如下:能明显增加正常小鼠脾重、白细胞总数及淋巴细胞数,对氢化可的松所致免疫功能抑制小鼠脾指数、白细胞总数和淋巴细胞数的降低有明显对抗作用;显著增强二硝基氯苯所致正常及环磷酰胺所致免疫抑制小鼠的迟发型过敏反应;增加正常小鼠外周血淋巴细胞 ANAE阳性百分率,并明显对抗氢化可的松所致的免疫抑制作用;但ⅡP体外实验对刀豆素 A 诱导的小鼠脾细胞淋转反应无明显增强作用:此外,ⅡP还能明显增强抗体形成细胞功能,增加小鼠静注碳粒廓清速率。
Polysaccharides extracted from the root of Isatis indigotica(IIP,50 mg/kg·d,ip×8d) significantly increased the weight of spleen and number of white blood cell and lymphocyte in peripheral blood in normal ICR mice,and antagonized the immunosuppressive actions of hydrocor- tisone.There were marked potentiating effects on delayed hypersensitivity reaction induced by 2, 4-dinitrochlorobenzene in normal NIH mice treated with IIP as well as in immunodepressed mice induced by cyclophosphamide. By administration of IIP,the percentage of ANAE^+ lymphocytes stained with acid α-naphthyl acetate esterase method were significantly increased in peripheral blood of normal ICR mice,and the decreases in number of ANAE^+ lymphocytes in mice induced by hydrocortisone were prevented to a certin extent.But IIP could not enhance Con A-induced Iymphocyt proliferation of C_(57)BL mouse spleen ceils in vitro measured with MTT colorimetric assay.In addition,the plaque forming cells in 5×10~6 splenocytes of NIH mice treated with IIP were higher than that of the control group (P<0.01).IIP could also elevate the clearance rate of intra-venous charcoal particles in normal mice,i. e.,stimulated the phagocytic activity of macro- phages.The result indicates that IIP is capable of increasing humoral and cellular immune functions and enhancing the functions of reticuloendothelial system,and might be a good immunopotentiator,
关键词
板蓝根
多糖
白细胞
淋巴细胞
脾脏
Isatis indigotica polysaccharide
immunostimulating effect
