摘要
本实验运用体外培养心肌细胞,观察了中药生脉注射液对阿霉素引起心肌细胞损伤时,心肌细胞内乳酸脱氢酶释放及超微结构的影响。结果表明,生脉注射液30、100、300mg/L 对阿霉素1 mg/L 作用4 h 引起的培养心肌细胞乳酸脱氢酶释放没有影响;对其引起的心肌细胞超微结构损伤也无改善作用。结果提示:生脉注射液对阿霉素引起的心肌损害的保护作用,可能是在整体情况下产生的。
The effect of Sheng Mai injection(SMI)on adriamycin-induced cardiotoxicity in cultured myocardial cells was examined.Adriamycin treatment(1 mg]L)for 4 h of culture age,increased remarkably the leakage of lactate dehydrogenase(LDH)from the myocardial cells,and induced the damage of ultrastructure.SMI(30 my/L,100 my/L,300 mg/L)had no effects on these changes induced by adriamycin.The results showed that SMI had no direct protective effect on adriamycin cardiotoxicity in cultured myocardial cells.It's protective effect on adriamycin cardiotoxicity seems to be in whole body level,the mechanism of which was relating to the metabolism of the drug in organism,and is worthy of further research.
基金
上海市青年自然科学基金资助
关键词
生脉注射液
阿霉素
心肌损害
预防
Sheng Mai injection
adriamycin
lactate dehydrogenase
ultrastructure
