摘要
[目的 ]Ames试验研究鞣酸和相关化合物抗突变作用。 [方法 ]应用平皿掺入和悬浮培养抗突变性方法 ,比较研究鞣酸和相关化合物在无抑制和抑制浓度减少 2 硝基芴 (2 NF)、4 硝基喹啉 1 氧化物 (4 NQO)和N 甲基 N 硝基 N 亚硝基胍 (MNNG)回复菌落数。 [结果 ]鞣酸和相关化合物月桂木立口 酸、鞣花酸、木立口 酸丙脂、木立口 酸甲脂、木立口 酸和儿茶素抑制Ames试验沙门氏菌菌株生长 ,这种抑制作用与减少Ames试验回复突变和诱发突变菌落数相关。应用悬浮培养方法 ,鞣酸和相关化合物在无抑制浓度和抑制浓度对 2 NF、4 NQO和MNNG无抗突变作用。 [结论 ]Ames试验悬浮培养法是研究抑菌剂类化合物抗突变有用方法 ,可区别由毒性和抗突变作用减少的回复菌落数。
To study the antimutagenic activity of tannic acid and related compounds in Ames assay. Using the standard plate incorporation and suspension culture antimutagenic methods,it was compared that tannic acid and related compounds at non inhibitory and inhibitory concentrations reduced the number of Salmonella tester strain revertants induced by 2 NF?4 NQO and MNNG. Tannic acid and related compounds such as lauryl gallate,ellagic acid,propyl gallate,methyl gallate,gallic acid and catechin were inhibitory to the growth of Ames Salmonella tester strains. Tannic acid and related compounds inhibitory potency was associated with reducing the number of Ames tester strain revertants. Employing the suspension cultural method,tannic acid and related compounds at non inhibitory and inhibitory concentrations against 2 NF,4 NQO,MNNG had shown no significant antimutagenic effect. [Conclusion] When Ames antimutagenicity assay for the compounds of inhibitory bacteria growth,suspension cultural method is useful,it can differentiate that the reduction of revertants is due to toxicity or antimutagenic effect of the compounds of inhibitory bacteria growth.
出处
《环境与职业医学》
CAS
北大核心
2002年第4期231-234,共4页
Journal of Environmental and Occupational Medicine
关键词
毒性
抗突变
鞣酸
AMES试验
toxicity
antimutagenicity
tannins
Ames assay
