摘要
以中国西门塔尔牛基因组DNA为模板 ,参照绵羊的FSHR基因序列设计和合成引物 ,PCR扩增获得长度为 931bp ,包括 5′端侧翼 797bp和结构基因第一外显子区 134bp的牛FSHR基因片段。将该片段克隆于PUC18载体中 ,作序列分析发现 :牛的FSHR基因转录启动子的遗传结构与人和鼠的FSHR基因不同 ,它的基因转录启动区含有TATA盒和类CAAT盒序列。在检索出的转录调节元件、或特征性序列位点 ,牛与绵羊在 5个序列位点有碱基变异 ,这可能与牛和绵羊的产仔率高低不同相关。对 34头份中国西门塔尔牛的扩增产物 ,应用PCR RFLP方法进行多态性分析 ,TaqⅠ酶切出现了多态性 ,酶切产物电泳呈现 3种基因型 ,由 2种等位基因构成。通过对基因频率和基因型频率在种用公牛、双胎母牛和随机牛类群中分布的比较研究结果 ,认为该基因与牛的繁殖性状存在连锁相关。
931 bp,Including 134 bp of the first exon and 797 bp 5′-flanking region,of bovine FSHR gene was amplified by PCR using primers designed according to ovine FSHR gene sequence.The PCR products were ligated into PUC18 vector and sequenced.It was found that the genetic components of the bovine FSHR promoter differ from those of rat and human FSHR gene promoter.There are TATA-box and CAAT-box-like sequences in the promoter of bovine FSHR gene.Base-differences also exist at 5 loci of transcriptional regulating elements or characteristic sequences searched for,which may relate to the litter size differences of cattle and sheep.We also studied the polymorphism of the PCR products of 34 Chinese Simmental by PCR-RFLP.2 alleles and 3 genotype were verified by endonuclease Taq Ⅰ.Distributive differences of allele and genotype frequencies among herd-bull,twin-calved cow and random-sampling cattle indicates that these alleles are linked or correlated with the reproductive traits of cattle.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2002年第5期417-423,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
