摘要
目的 :探索诊断过敏性休克的体外检测方法。方法 :建立豚鼠过敏性休克模型 ,培养RBL 2H3细胞株 ,用致敏动物血清在体外诱导该细胞脱颗粒 ,观察其形态改变及测定组胺释放量与细胞表面AnnexinV标记阳性率。结果 :RBL 2H3细胞在致敏血清刺激下活化 ,在抗原再次攻击后可以脱颗粒 ,AnnexinV阳性率与组胺释放量呈正比 ,二者与致敏血清的滴度呈剂量依赖关系。结论 :RBL 2H3可望用于过敏性疾病诊断及变应原筛选 。
Objective:In order to study of diagnose anaphylactic shock in forensic field,a new in vitro testing sysytem were established.Methods:The RBL 2H3 were cultured and sensitized,the release of histamine and stained for Annexin V were testing by fluorescent spectrometer and flow cytometry respectively.Results:The degree of RBL 2H3 degranulation after sensitived serum treated was higher than that after control serum treated,and the degranulation degree increased dose dependently.Conclusion:RBL 2H3 are helpful to diagnose anaphylactic shock,and the release of histamine and the positive staining for Annexin V may be the ideal indices.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2002年第9期611-613,共3页
Chinese Journal of Immunology
