摘要
目的 了解P16、PRb在肝癌中的表达及其在肝癌发生、发展中的作用。方法 用免疫组化S—P法 ,对46例肝癌及癌旁组织和 10例正常肝组织进行对比研究。结果 (1) 46例肝癌组织中P16及PRb的阳性率分别为45 .6%和 5 6.5 % ,均明显低于相应癌周组织 89%、91.3 %和正常组织 10 0 %的水平 (P <0 .0 1) ;(2 )P16与PRb呈负相关 ;(3 )P16与肿癌的病理分级、门静脉癌栓及肿瘤包膜密切相关 ;PRb仅与前者有关。结论 (1)P16、PRb在肝癌中有较高的缺失率 ,可能与肝癌的发生、发展关系密切 ;(2 )P16与PRb呈负相关 ,验证了P16与PRb在细胞周期调节中的负反馈学说 ;(3 )P16、PRb与肝癌的大小、病理分级、门静脉癌栓及具有完整包膜等因素有密切关系 ,可考虑将其作为评价肝癌预后的指标。
Objective To study the expression of P16,PRb and their clinical pathological significances,carcinogenesis and progress in hepatocellular carcinoma.Method Immuohistochemical (S-P) technique was used to detect the expression of P16,PRb in the 46 patients with hepatocellular carcinoma and 10 cases with normal control.Results (1) In the patients with hepatocellular carcinoma,the expressive rate of P16 and PRb in the tumoral tissue were45.6%(21/46) and 56.5%(26/46), which were significantly lower than that in adjacent non-tumorous tissue and normal tissue. (2) The expression of P16 was inversely related to the expression of PRb in tumoral tissue. (3) There was singnificant relationship between the expression of P16 and tumor capsula,differentiation and thrombus in the portal vein.The expression of PRb was related to the tumor differentiation and thrombus in the portal vein.Conclusions (1) The lower expression of P16,PRb in tumoral tissue could related to the carcinogenesis and progress of HCC. (2) The inversely relationship between P16 and PRb conformed the hypothesis of feedback in cell cycle.(3) The relationship between the expression of P16, PRb and tumoral parameters may imply that they can serve as an indicator in evaluating the prognosis of HCC.
出处
《安徽医学》
2002年第5期18-20,共3页
Anhui Medical Journal
关键词
肝癌
P16
PRB
免疫组化
Hepatocellular carcinoma
P16
Retinoblastoma protein
Immunohistochemistry
