摘要
以柴胡皂苷A ,芍药苷 ,橙皮苷 ,甘草酸四种指标成分的含量为指标 ,考察复方柴胡汤大孔吸附树脂精制工艺。结果选用 1倍 (生药 )量S型大孔树脂 ,以 1倍量水洗 ,4倍 (生药量 ) 70 %乙醇洗脱树脂为最佳工艺 ,大孔树脂反复使用 4 0次 ,干膏率、橙皮苷及芍药苷含量均无下降趋势。
The purification conditions by absorption resins were optimized by content determination of saikosaponin A,paeoniforin,hesperidin and glycyrrhizic acid.S type synthetic absorption resin was selected.The optimal purification conditions were:specific input sample amount(ratio of weight of medical raw material to volume of resin,W/V)was one,ltime of water to elute the resin and collecting 4 times of 70% ethanol(V/W) as elution solvent.The yield and contents of paeoniforin and hesperidin of dried extract,purified by the absorption resin used repeatedly for 40 times,was not decreased compared with the new resin.
出处
《中国实验方剂学杂志》
CAS
2002年第5期1-3,共3页
Chinese Journal of Experimental Traditional Medical Formulae
