摘要
目的综述肌酸激酶的结构与功能研究进展以及应用。方法根据近年来的文献 ,介绍了肌酸激酶的结构、在不同条件下伴随构象变化其功能的改变机制、生理功能的研究进展以及实际应用。结果肌酸激酶是研究蛋白质体内折叠的机制的理想模型 ,已经取得了突破性的进展 ,为生物大分子的结构与功能理论提供了依据。肌酸激酶具有重要的生理功能 ,目前在临床如肿瘤检测方面已有广泛的应用。结论对肌酸激酶的结构与功能的研究 ,将会广泛地应用于临床诊断、新药研究等方面。
Objective This article reviewed the progress in application of creatine kinase and the relationship between the structure and functions. Methods The structure and the relationship between its activation and conformation changes under different conditions were mainly introduced according to the documents in recent years. Here we also reviewed the advance of studies on its physiological roles and its application. Results CK is an ideal model for studying the unfolding and refolding of protein in vitro . The mechanisms of folding will be helpful to understanding the theory of the relationship between the structure and function of high molecules. In addition to its physiological role, CK is important in the diagnoses of some diseases such as clinical tumor specimens. Conclusions It will be widely used on clinical diagnoses and discoveries of new drugs depending on the study of the relationship between its structure and functions.
出处
《沈阳药科大学学报》
CAS
CSCD
2002年第5期386-390,共5页
Journal of Shenyang Pharmaceutical University
关键词
肌酸激酶
构象
变性
复性
研究进展
creatine kinase
conformation
denaturation
renaturation
