摘要
目的 探讨＋ ＧＺ作用后，大鼠心肌组织内内皮素－１（ＥＴ－１）和内皮型一氧化氮合酶（ｅＮＯＳ）ｍ ＲＮＡ 表达变化。 方法 ２４只雄性Ｗｉｓｔａｒ 大鼠随机分成对照组、＋ ＧＺ重复暴露后３０ ｍ ｉｎ、６ ｈ 和２４ ｈ四组，每组６只。对照组大鼠Ｇ 值为＋ １ ＧＺ， 实验组大鼠在动物离心机上经历了３次＋ １０ ＧＺ／３ ｍ ｉｎ（两次中间间隔３０ ｍ ｉｎ）作用。分别于暴露后３０ ｍ ｉｎ、６ ｈ 和２４ ｈ 处死大鼠取心，提取总ＲＮＡ，用半定量反转录聚合酶链反应（ＲＴ－ＰＣＲ）检测方法检测大鼠心肌组织ＥＴ－１和ｅＮＯＳｍ ＲＮＡ 表达水平。 结果 ＋ ＧＺ暴露后３０ ｍ ｉｎ 和６ ｈ 大鼠心肌组织ＥＴ－１ ｍ ＲＮＡ 明显升高，ｅＮＯＳｍ ＲＮＡ 明显降低，但２４ ｈ 时均恢复正常。 结论 ＋ ＧＺ暴露可使大鼠心肌组织内ＥＴ－１和ｅＮＯＳｍ ＲＮＡ 表达发生改变，其在心肌损伤中可能起一定的作用。
To explore the changes of mRNA expression of endothelin 1 (ET 1) and endothelial nitric oxide synthase (eNOS) in the heart of rat exposured to repeated +G Z. Methods Twenty four concious Wistar rats served as subjects. They were randomly divided into 4 groups. Using an animal centrifuge, control rats ( n =6) were exposed to +1 G Z and experimental rats ( n =18) were exposed to +10 G Z three times, each for 3 min with 30 min interval in between. The hearts were taken 30 min, 6h and 24 h after the last centrifuge run and total RNA was isolated by single step acid guanidinium thiocyanate phenol chloroform extraction method. The mRNA expression levels of ET 1 and eNOS in rat heart were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR). Results The ET 1 mRNA expression levels in rat hearts taken 30 min and 6 h after repeated +G Z exposures were significantly higher than those of control group, the eNOS mRNA levels were significantly lower, but the differences between the levels of control group and those of experimental rat heart taken 24 h after +G Z exposure were non significant. Conclusion It is suggested that ET 1 and eNOS mRNA expression in rat heart can be altered by repeated +G Z exposures and the altered expression of ET 1 and eNOS may play a role in the pathologic course of heart damage induced by+G Z exposures, but the changes were reversiable.
出处
《空军总医院学报》
1998年第4期196-199,共4页
Journal of General Hospital of Air Force,PLA
