摘要
目的 :大鼠灌服 99Tcm -嗜酸乳杆菌后 ,观察肠影像变化 ,测定小肠传递时间。方法 :将 18只大鼠随机分 3组 ,每组 6只。第一组动物灌 99Tcm-嗜酸乳杆菌 ;第二组动物灌 99Tcm O- 41mci;第三组动物灌 99Tcm- DTPA面粉湖剂 1m ci。灌胃后 1、2、3h,分别做平面静态显像。 3.5 h,断颈处死动物 ,取血计算毫升数 ,解剖取胃、小肠、大肠和盲肠。测定每只动物血中的放射性计数。测定胃、小肠、大肠和盲肠的微居里(μci)数。结果 :3组动物肠影像无明显差异。随着灌胃后时间的延长 ,小肠影像逐渐变大 ,放射性分布逐渐浓聚。小肠内放射性活度 ,第一组和第三组分别与第二组比放射性活度高 ,差异有显著性 (P<0 .0 1) ,第一组与第三组比相似 ,差异无显著性 (P>0 .0 5 )。大肠内放射性活度 ,第一组和第三组分别与第二组比放射性活度低 ,差异有显著性 (P<0 .0 1) ,第一组与第三组比相似 ,差异无显著性 (P>0 .0 5 )。结论 :运用 99Tcm-乳杆菌测定小肠传递时间除具有传统方法测定所具有作用外 ,还由于乳杆菌在胃肠道内突出的生理作用 ,必将在抗生素的利用、控制肠道感染、控制血清胆固醇水平、免疫和抗肿瘤方面的研究发挥重要作用 。
Objective:Small bowel imaging is observed and small bowel transit time (SBTT)determined.Methods:18 wistar rats were randomly separated into 3 groups and 6 rats per group.The rats of group 1 were perfused with 99 Tc m-LA 1 mci,the ones of group 2 using 99 Tc mO - 41mci,and the ones of group 3 with 99 Tc m-diethylenetriamine pentaacetic acid(DTPA)flour paste 1 mci.The rats of 3 groups were all imaged staticly with SPECT respectively 1h,2h,and 3h after perfused.We killed all the rats by cutting their cervices 3h after perfused,at the same time we collected their blood,counted the ml number of the blood,anatomized and collected their stomachs,small intestines,large intestines and caeca.Radioactive counts of the blood per ml per rat were assaied.Radioactivity(μci)per stomach,small intestine,large intestine and caecum was detected.Results:There was no obvious difference among images of all intestine,which shapes were like a circle,ellipse,stick and et al.The longer it was after all the rats were perfused,the larger the intestines'images became.At the same time the radioactive distribution in the intestines was growing dense.The radioactivity in the first and third groups'intestines increased significantly,respectively compared with that of the second group's ( P <0 01),but that of the first compared with that of the third was similar( P >0 05).That in the first and third groups'large intestines decreased,respectively compared with that of the second group's( P <0 01).That of the first compared with that of the third was similar( P >0 05).Conclusions:SBTT with 99 Tc m-LA not only has the same effect as with traditional method (group 3),but that will also play a significant part in the study of the use of antibiotic ,the control of intestinal infection and serum cholesterol,immunity and antitumor thanks to LA's prominent function in the intestinal tract,at the same time we can get the image of the intestine also.
出处
《中国微生态学杂志》
CAS
CSCD
2002年第4期196-198,共3页
Chinese Journal of Microecology
