摘要
应用放射免疫学方法 ,检测腹腔注射二甲苯胺噻嗪后大鼠血浆、下丘脑和垂体中 β-内啡肽 (β- Ep)含量的变化 ,以研究二甲苯胺噻嗪对大鼠体内β- Ep的影响。结果 ,不同时间组与对照组相比 ,血浆在给药后 5 min即有统计学意义(P <0 .0 1) ,大约在 30 m in时达到最高峰 ,然后缓慢下降 ,其中麻醉组持续到给药后 12 0 min(P <0 .0 1) ,而镇痛组只持续到给药后 90 min。不同剂量组与对照组相比 ,下丘脑的 4mg/kg组无显著性差异 (P >0 .0 5 ) ,血浆的 4mg/kg组、垂体的 4mg/kg组差异有显著意义 (P <0 .0 5 ) ,其他组有极显著意义 (P <0 .0 1)。随着给药剂量的增加 ,组织内的β- Ep含量变化呈增加的趋势 ,这种趋势经相关分析表明 ,给药剂量在 4～ 6 0 mg/kg的范围内时 ,组织内的β- Ep含量与给药剂量呈正的直线相关关系 (r血浆 =0 .9748,r下丘脑 =0 .96 7,r垂体 =0 .977,均 P <0 .0 1)
This study was carried out to investigate the mechanism of analgesic and anesthetic activity of xylazine. Radioimmunoassay was used to measure the change of beta-endorphin(β-Ep) in the blood, hypothalamus and pituitary gland when xylazine was given. The rats were assigned to 3 groups and treated as follows: saline control, analgesic groups(25 mg/kg), anesthetic groups(55 mg/kg). The blood β-Ep level of analgesic groups and anesthetic groups increased significantly at 5 min (P<0.01) , reached the top level at 30 min (P<0.01), and then decreased gradually until 90 min (analgesic groups, P<0.01) or 120 min (anesthetic groups, P<0.01). But the highest level in hypothalamus and pituitary gland occured 15 min prior to the top level in blood. In the different dose-treated groups, the level of β-Ep in blood, hypothalamus and pituitary gland increased with the dose given correlatively (P<0.01). It is inferred that xylazine may enhance the synthesis and excretion of beta-endorphin in hypothalamus and pituitary gland.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2002年第5期503-505,共3页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目 ( 396 70 5 6 7)
