摘要
本文报道了浓度为 0 0 5～ 2 0mM的重金属Cu、Cd、Hg胁迫对 3个不同类型水稻品种幼苗叶片过氧化氢酶 (CAT)活性和同功酶表达的影响。结果表明 :( 1 )在胁迫浓度为 0 0 5～ 2 0mM范围内 ,CAT活性随Cu和Cd浓度的增高而持续下降 ;CAT活性随Hg浓度的增高表现为先下降 ,再略有升高 ,然后又明显下降 ,表明浓度为 0 0 5～ 2 0mM的Cu、Cd、Hg胁迫显著抑制 3个不同类型水稻品种幼苗叶片CAT活性。( 2 )应用聚丙烯胺浓度梯度电泳对CAT同功酶分析显示 ,正常情况下水稻叶片中有 1～ 2个CAT同功酶的表达 (武育粳叶片中表达 2个 ,扬稻 6号和汕优 81 8叶片中表达1个 )。 0 1mM的Cd胁迫能诱导武育粳叶片 ,0 1mM的Cd和Hg胁迫能诱导扬稻 6号叶片表达 1个新的CAT同功酶。尽管如此 ,正常表达的CAT同功酶仍受到重金属Cu、Cd。
The effect of heavy metal stress on the catalase(CAT) acivity and expression of isozymes in the leaves of rice(Wuyujing,Yangdao 6\,Shanyou 818) seedling was measured and analyzed.The results showed as follows.(1)When the concentration of Cu,Cd and Hg was in the range of 0\^05～2\^0mM,the CAT activity decreased continuously with the concentration of Cu and Cd increasing.However,with the concentration of Hg increasing the CAT activity rapidly decreased first,and then increased slightly,and again decreased obviously,indicating that the Cu,Cd and Hg of 0\^05～2\^0mM inhibited the CAT activity in the leaves of rice seedling.(2)The results by using polyacrylamide concentration gradient gel electrophoresis technique to analyze the CAT isozymes indicated that,on the normal condition,there were 1 to 2 CAT isozymes being expressed in the rice leaves(2 CAT isozymes being expressed in Wuyujing leaves, 1 CAT isozymes in Yangdao 6 and Shanyou 818 leaves).0\^1mM Cd stress induced Wuyujing leaves to express 1 new CAT isozymes,0\^1mM Cd and Hg stress also induced Yangdao 6 leaves to express 1 new CAT isozymes,but the expression of CAT isozymes,which were expressed in normal condition,were inhibited by Cu,Cd and Hg stress.
出处
《核农学报》
CAS
CSCD
北大核心
2002年第4期197-202,共6页
Journal of Nuclear Agricultural Sciences
