摘要
采用PCR-限制性片段长度多态性分析法(PCR-RFLP)检测47例人肺癌P53基因249位密码子点突变。结果显示,NSCLCP53基因249密码子突变率24.24%(8/33),SCLC突变率为0(0/14),P53基因249位密码子点突变与肺癌分期、组织分化、吸烟无关(P>0.05),与NSCLC淋巴结转移相关(P<0.05)提示:P53基因249位密码子突变是NSCLC突变热点,是评估NSCLC预后因素之一。
47 Case of P53 gene 249 codon mulations of human lungcancer were detected by PCR-RFLP.The results showed that P53 gene 249codon mutation was 24.24%(8/33)in NSCLC,Mutation rate was 0(0/14)in SCLC.There didn't appear to be an association between this mutation andstaging,histological differentiation,Smoking in lung cancer(P>0.05)P53 gene 249 codon mutation correlated with metastatic lymph nodes in NSCLC(P<0.05).These observations suggest that P53 gene 249 codonmutation is a hotpoint in NSCLC,also is to assess one of the prognosticfactor for NSCLC.
出处
《空军总医院学报》
1996年第3期138-140,共3页
Journal of General Hospital of Air Force,PLA
