摘要
目的:研究弥漫大B细胞淋巴瘤(DLBCL)中己糖激酶-Ⅱ(HK-Ⅱ)、肿瘤坏死因子α诱导蛋白3基因(TNFAIP3)异常表达与肿瘤细胞恶性特征的相关性。方法:选择2016年3月～2018年3月期间在我院手术切除的DLBCL患者作为DLBCL组,同期手术切除且经病理证实为淋巴结反应性增生的患者作为对照组,收集病灶后测定HK-Ⅱ、TNFAIP3、增殖基因、侵袭基因的表达量。结果:DLBCL组患者病灶内HK-Ⅱ的mRNA表达量明显高于对照组,TNFAIP3的mRNA表达量明显低于对照组;DLBCL组淋巴瘤分期Ⅲ～Ⅳ期、淋巴瘤分组B组患者病灶内HK-Ⅱ的mRNA表达量明显高于I～Ⅱ期患者、A组患者,TNFAIP3的mRNA表达量明显低于I～Ⅱ期患者、A组患者;DLBCL组患者病灶内CyclinD2、PDE4B、BCL2、XIAP、CCL5、CXCR4、MMP26的mRNA表达量明显高于对照组且与HK-II呈正相关、与TNFAIP3呈负相关,Caspase-3、TIMP4的mRNA表达量明显低于对照组且与HK-Ⅱ呈负相关、与TNFAIP3呈正相关。结论:DLBCL中HK-Ⅱ的高表达以及TNFAIP3的低表达与肿瘤病理进程、肿瘤细胞增殖及侵袭密切相关。
Objective： To study the correlation of the abnormal expression of hexokinase-Ⅱ （HK-Ⅱ） and tumor necrosis factor alpha-induced protein 3 （TNFAIP3） and the malignant features of tumor cells in diffuse large B-cell lymphoma （DLBCL）. Methods： DLBCL patients who underwent surgical resection in our hospital between March 2016 and March 2018 were selected as the DLBCL group, and the patients who underwent surgical resection and were pathologically confirmed as reactive hyperplasia of lymph nodes during the same period were selected as the control group. The lesions were collected to measure the expression levels of HK-Ⅱ, TNFAIP3, proliferation genes and invasion genes. Results： HK-Ⅱ mRNA expression level in the lesions of the DLBCL group was significantly higher than that of the control group while TNFAIP3 mRNA expression level was significantly lower than that of the control group; In DLBCL group, HK-Ⅱ mRNA expression levels in the lesions of patients with lymphoma stage Ⅲ-Ⅳ and lymphoma group B were significantly higher than those of the patients with lymphoma stage I-Ⅱ and lymphoma group A while TNFAIP3 mRNA expression levels were significantly lower than those of the patients with lymphoma stage I-Ⅱ and lymphoma group A; In the lesions of the DLBCL group, CyclinD2, PDE4B, BCL2, XIAP, CCL5, CXCR4 and MMP26 mRNA expression levels were significantly higher than those of the control group, and were positively correlated with HK-Ⅱ and negatively correlated with TNFAIP3; while Caspase-3 and TIMP4 mRNA expression levels were significantly lower than those of the control group, and were negatively correlated with HK-Ⅱ and positively correlated with TNFAIP3. Conclusions： The high expression of HK-Ⅱ and the low expression of TNFAIP3 in DLBCL are closely related to the pathological process of tumor as well as the proliferation and invasion of tumor cells.
作者
秦英
QIN Ying(Hematology Department,Ya＇an People＇s Hospital in Sichuan Province,Ya＇an 625000,China)
出处
《海南医学院学报》
CAS
2018年第22期2015-2018,2022,共5页
Journal of Hainan Medical University
基金
四川省科技攻关计划基金项目(2003sz0398)~~
