摘要
本文研究西藏地区藏猪源流感病毒(SIV)H1、H3亚型血清抗体情况。2016年11月从西藏拉萨、林芝、日喀则、山南和昌都共采集202份藏猪血清样品,利用酶联免疫吸附试验(ELISA)检测血清样品中猪流感H1、H3两个亚型血清抗体存在情况,并用SPSS 22软件对不同地区的抗体阳性率进行统计分析。结果显示,藏猪流感H1亚型血清抗体总阳性率为50.99%(202/103),H3亚型血清抗体总阳性率为46.04%(202/93);从不同地区来看,藏猪源流感H1、H3两个亚型血清抗体阳性率不存在显著差异(P>0.05)。结果说明猪流感病毒在西藏地区仍仍存在较高的流行率,在藏猪猪病防控中必须重视。本研究较为全面的调查了西藏地区猪流感病毒H1、H3抗体在藏猪血清样品中的阳性率,为西藏地区藏猪流感疾病的研究积累了资料并提供了参考。
This paper studied the state of serum antibody against Tibetan swine influenza virus（SIV） H1,H3 subtype infection in Tibetan. A total of 202 swine serum samples were collected from Lhasa, Nyingchi, Shigatse, Shannan and Qamdo prefectures in Tibet, and the presence of antibodies against SIV H1, H3 subtype were tested by enzyme-linked immunosorbent assay（ELISA）, and the positive rates of antibody in different regions were analyzed by SPSS 22. The results showed that the total positive rate of serum antibody of swine influenza H1 subtype was 50.99%（202/103）, and the total positive rate of antibody of H3 subtype was 46.04%（202/93）. There was no significant difference（P〈0.05） in the positive rates of the two subtypes in different regions. This result indicated that there was still a high prevalence of swine influenza virus in Tibet, which must be emphasized in the prevention and control of Tibetan swine disease. In summary, we thoroughly investigated the epidemiological situation of swine flu（H1, H3） in Tibet swine, which can provide data and reference for prevention and control of Tibetan swine flu diseases.
作者
贡嘎
王一飞
程少龙
索朗斯珠
崔爱莲
GONG Ga;WANG YiFei;CHENG ShaoLong;SUO Lang;Si Zhu;CUI AiLian(Animal Science College,Tibet Agriculture ＆ Animal Husbandry University,Nyingchi Tibet,860000,China;Animal Science College,HuaZhong Agricultural University,WuhanHubei,430070)
出处
《高原农业》
2017年第2期139-142,共4页
Journal of Plateau Agriculture
基金
藏猪流感病毒分子流行病学调查研究(QCZ2016-51)
