摘要
目的定性、定量分析三叶青叶黄酮类化学成分。方法采用超高效液相色谱-串联质谱(UPLC-MS/MS)对三叶青叶化学成分进行鉴定,色谱柱为C_(18) Luna Omega(2.1 mm×100 mm,1.6μm);流动相:乙腈-水(50∶50,V/V);流速0.4 mL/min;柱温35℃;进样量5μL;全扫描模式扫描。采用高效液相色谱法(HPLC)同时测定三叶青叶中5种碳苷黄酮的含量。色谱柱为Ultimate XB C_(18)(250 mm×4.6 mm,5μm);流动相为乙腈-0.2% H_3PO_4(10∶90,V/V);流速为1 mL/min;检测波长为350 nm;室温;进样量20μL。结果三叶青叶中共鉴定出6个化学成分,其中5个黄酮成分的保留时间、光谱图和质谱碎片离子信息与对照品一致,分别为异荭草素、荭草素、牡荆素鼠李糖苷、牡荆素、异牡荆素。HPLC测定8个不同产地来源的样品中上述5种成分含量,各成分线性关系良好(r> 0.9997),加样回收率在95.0%～105.0%之间。结论三叶青叶子中主要含2类碳苷黄酮,即荭草素类和牡荆素类;本研究建立的方法可定性、定量分析分析三叶青叶中主要化学成分,可用于控制药材质量。
Objective Qualitative and quantitative analysis of the chemical constituents of flavonoids in the leaves of Tetrastigma hemsleyanum. Methods The chemical constituents in leaves of Tetrastigma hemsleyanum were identified by ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. The column was C18 Luna Omega （2.1 mm × 100 mm, 1.6 μm）; the mobile phase was acetonitrile-water （50∶50, V/V）; the flow rate was 0.4 mL/min; the column temperature was 35°C; the injection volume was 5 μL; full scan mode. The contents of five carbon glycosides in the leaves of Tetrastigma hemsleyanum were determined at the same time by high performance liquid chromatography （HPLC）. The column was Ultimate XB C18 （250 mm×4.6 mm, 5 μm）; the mobile phase was acetonitrile - 0.2% H3PO4 （10∶90, V/V）; the flow rate was 1 mL/min; the detection wavelength was 350 nm; room temperature; injection volume was 20 μL. Results A total of 6 chemical components were identified in the leaves, and the retention time, spectrum and mass spectrometry information of the 5 flavonoids were consistent with the control, which were isohumullin, valerin, vitexin and rhamnoside, vitexin, isovite. The contents of the above five components in the samples from 8 different origin sources were determined by HPLC. The linear relationship of each component was good （r 〉 0.9997）, and the recovery rate was between 95.0% and 105.0%. Conclusion The leaves of Tetrastigma hemsleyanum are mainly composed of two kinds of carbon flavonoids, valerin and vitexin. The method established in this study can qualitatively and quantitatively analyze the main chemical components in the leaves of Tetrastigma hemsleyanum and control the quality of the medicinal materials.
作者
邓思珊
刘洪旭
马丽红
汪婷婷
王亚端
黄心萍
DENG Sishan;LIU Hongxu;MA Lihong;WANG Tingting;WANG Yaduan;HUANG Xinping(Fujian Key Laboratory of Medical Measurement,Fujian Academy of Medical Sciences,Fujian Province,Fuzhou,350001,China;School of Pharmacy,Fujian Medical University,Fujian Province,Fuzhou,350108,China;Fuzhou Jizhenyuan Biological Technology Co.,Ltd.,Fujian Province,Fuzhou,350001,China)
出处
《中国医药导报》
CAS
2018年第33期80-84,88,共6页
China Medical Herald
基金
福建省省属公益类科研院所基本专项(2015R1031-3)
福建省自然科学基金资助项目(2016J01351)
关键词
三叶青
黄酮类
超高效液相色谱-串联质谱
高效液相色谱
Tetrastigma hemsleyanum
Flavonoids
Ultra performance liquid chromatography-tandem mass
High performance liquid chromatography
