摘要
制备了粒径为13nm的金纳米粒子,在其表面修饰双酚A(BPA)适配体作为能量受体探针;并利用聚丙烯酸(PAA)包覆油溶性的上转换荧光纳米材料(UCNPs)制备水溶性的UCNPs,在其表面修饰适配体互补链形成功能化UCNPs作为能量供体,构建了基于FRET原理的BPA生物传感检测平台。结果表明:该检测体系在1×10^(-9)～1×10^(-3) mol/L时具有良好的线性关系(R^2=0.992 3),检出限低至1×10^(-10) mol/L。
The 13 nm gold nanoparticles were synthesized and were modified with Bisphenol A（BPA）aptamer.The oil-solubility Upconversion nanoparticles（UCNPs）were modified with polyacrylic acid（PAA）to form water-solubility UCNPs.Then water-solubility UCNPs were coated with the complementary DNA strand.Based these nanoparticles,a FRET aptasensor for BPA was successfully fabricated by using Up-conversion nanoparticles as energy donor and gold nanoparticles as energy acceptor.The results show that the detection system has a good linear relationship at 1×10^（-9）～1×10^（-3） mol/L（R^2=0.992 3）,and the detection limit is as low as 1×10^（-10） mol/L.The method is proved to be of good practicability through the experiment of adding the standard of water and milk samples.
作者
许宙
鲁士珍
陈茂龙
朱颖越
丁利
程云辉
XU Zhou;LU Shi-zhen;CHEN Mao-long;ZHU Ying-yue;DING Li;CHENG Yun-hui(School of Chemistry and Biological Engineering,Changsha University of Science ＆ Technology,Changsha,Hunan 410114,China;School of Biotechnology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 215500,China)
出处
《食品与机械》
CSCD
北大核心
2018年第9期83-87,共5页
Food and Machinery
基金
国家自然科学基金(编号:31401566)
国家重点研发计划(编号:2016YFF0203701)
粮食深加工与品质控制湖南省2011协同创新项目
常熟市科技发展计划(社会发展类)项目(编号:CS201605)
苏州市科技计划项目(编号:SNG201617)
关键词
荧光共振能量转移
上转换荧光纳米材料
金纳米颗粒
双酚A
fluorescence resonance energy transfer
Up-conversionnanoparticles
gold nanoparticles
bisphenol A
