摘要
水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)由介体灰飞虱以持久性不经卵方式传播。该病毒引起的水稻黑条矮缩病分布范围广、危害重。RNA干扰(RNA interference,RNAi)是近年来发现的一种重要的植物抗病毒基因工程策略,已广泛应用于植物抗病毒研究中。本研究利用已构建的包含P10 CP基因部分片段反向重复序列和生物安全性更高的标记基因bar基因的RNAi双元表达载体pRBSDV-CP3-bar,采用农杆菌介导转化水稻愈伤组织获得抗黑条矮缩病和抗除草剂的转基因水稻植株,通过抗性筛选、PCR检测及分子杂交分析,获得2个抗RBSDV和Basta的稳定品系,并对其田间抗性及农艺性状表现进行了鉴定,为抗RBSDV水稻新品种(品系)的培育奠定了基础。
Rice black-streaked dwarf virus （RBSDV） is transmitted by the small brown planthopper （SPBH, Laodelphax striatellus Fallen） persistently. Rice black-streaked dwarf disease is one of the most wide spread and severe virus diseases. RNA interference （RNAi） is an important gene silencing phenomenon and has been widely used in the transgenic breeding for improving crops viral resistantce. According to the principle of RNA interference mechanism, a new RNAi binary vector named pRBSDV- CP3-bar, was constructed including the inverted-repeat sequence of P10 CP gene partial fragment and the marker gene bar with higher biosecurity,and was used to transform rice callus mediated by Agrobacterium in order to obtain anti-RBSDV and herbicide-resistant transgenic rice plants. After analyzed by PCR and northern blot,two anti-basta and highly RBSDV-resistant rice varieties KRBSDV2 and KRBSDV6 were obtained. Their performance of field resistance and agronomy traits were investgated. It laid a foundation for the breeding of new rice varieties （lines） resistant to RBSDV.
作者
朱文银
徐建第
杨连群
姜明松
Zhu Wenyin;Xu Jiandi;Yang Lianqun;Jiang Mingsong(Shandong Rice Research Institute,Jinan 250100,China;Biotechnology Research Center,Shandong Academy of Agricultral Sciences,Jinan 250100,China)
出处
《山东农业科学》
2018年第11期23-27,共5页
Shandong Agricultural Sciences
基金
国家科技支撑计划子课题(2015BAD01B02-1-3)
国家重点研发计划子课题(2017YFD010050503)
山东省农业科学院农业科技创新工程项目(CXGC2016A11
CXGC2018B04)
关键词
水稻黑条矮缩病毒
RNA干扰
反向重复序列
农杆菌介导
Rice black-streaked dwarf virus
RNA interference
Inverted-repeat sequences
Agrobacterium- mediated
